原桃胶中1株芽孢杆菌的分离鉴定及其主要抗菌物质-河南农业科学.pdfVIP

136 2016, Vol.37, No.21 食品科学 ※生物工程 原桃胶中1 株芽孢杆菌的分离鉴定及其 主要抗菌物质 贾 锐，陆兆新* （南京农业大学食品科技学院，江苏南京 210095 ） 摘  要： 1 TJ12 Staphylococcus 从天然原桃胶分离到 株产抑菌活性物质的芽孢杆菌 。其发酵产物对金黄色葡萄球菌（ aureus ）、藤黄微球菌（Micrococcus luteus ）、蜡样芽孢杆菌（Bacillus cereus ）和串珠镰刀菌（Fusarium moniliforme 16S rRNA gyrB TJ12 ）有抑菌活性。结合菌落形态、生理生化指标、 序列和 序列将 菌株鉴定为地衣芽孢杆 菌（Bacillus licheniformis ）。通过硫铵沉淀、有机溶剂萃取、羟丙基葡聚糖凝胶、高效液相色谱分离纯化，得到其 pH 3 K 主要活性物质。该物质对 值、温度和 种蛋白酶（胰蛋白酶、蛋白酶 、胃蛋白酶）有较好的稳定性。经电喷雾 TJ12 A 电离质谱分析，初步鉴定该株地衣芽孢杆菌 的主要抗菌物质为杆菌肽 。 关键词：原桃胶；地衣芽孢杆菌；抗菌物质；分离纯化；鉴定；杆菌肽A Isolation and Identification of a Bacillus from Peach Tree Gum and Its Main Antimicrobial Substance * JIA Rui, LU Zhaoxin (College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China) Abstract: A strain of Bacillus named as TJ12 was isolated from peach tree gum, which could produce antibacterial substances. Its fermentation products had antibacterial activity against Staphylococcus aureus, Micrococcus luteus, Bacillus cereus and Fusarium moniliforme. Through colony morphology, physiological and biochemical characterization and 16S rRNA and gyrB sequence analysis, the strain was identified as Bacillus licheniformis . The major bioactive substance was separated from its fermentation supernatan