哮喘中il5和il10基因表达及其相关性研究.pdfVIP

2000, 25( 2) B LL H NAN MED NIV 99 IL5 IL10 罗百灵, 吴鄂生, 丘万服, 海镇环 附属湘雅医院内科( 长沙 410008) [ ] 31 只健康 鼠分为哮喘组地塞米松干预组及对照组测定各组 鼠肺泡灌洗液细胞成分, 应用RT PCR 方法检测支气管组织白细胞介素5( IL5) 和白细胞介素10(IL10)mRNA 表达强度结果显示, 哮喘组 鼠肺泡灌 洗液中嗜酸性粒细胞(EOS)百分比IL5 mRNA 表达量均显著高于地塞米松治疗组及对照组, 而IL10 mRNA 表达则相 反IL5 mRNA 表达与EOS 百分比呈正相关,而与 IL10 mRNA 表达呈负相关说明EOS,IL5以正相关参与了哮喘发 病哮喘时IL10表达受抑制,这可能是炎症细胞因子 IL5 合成增多的主要原因之一糖皮质激素治疗哮喘的作用 机制之一, 可能与其提高IL10 及下调IL5表达水平有关 [ ] 支气管疾病; 白细胞介素5; 白细胞介素10; 嗜酸性粒细胞; 多聚酶链反应 [ ] R56225 [] A [ ] 2000 Expression of interleukin5 and interleukin10 and their relationship in asthma L O Bailing, W Esheng, QI Wanfu, HAI Zhenhuan D epartment of I nternal Medicine , X iangy a H osp ital , Hunan Medical Univers ity ( Changsha 410008) [ Abstract] To explore the expression of interleukin5( IL5) and interleukin10( IL10) and their rela tionship in asthma, we measured the cells in bronchial aveolar lavagefluid(BALF) of guineapigs, and examined the expression of IL5mRNA and IL10 mRNA of bronchialtissue by reversetranscription polymerase chain reac tion(RTPCR) in control group(Group C, n = 10) , asthmatic group( Group A, n = 11) and dexamethasone treatment group(Group B, n = 10) . The results showed that the eosinophil(EOS) percentage and the expression of IL5 mRNA were significantly higher in Group A[(48. 64 17. 8) % , ( 106.91 20. 09) % respectively] than that in Group C[( 15. 10 11.48) %, (62.60 13.84) %] and Group B[(28.4 10.32) % , (75.30 10.70) %] . In constrast, the IL10 mRNA expression in Group A[(47.5 5.3) %] was obviously lower com pared with that in Group C[( 101.2 4.2) %]