不同培养条件对勺叶茅膏菜试管苗矶松素含量的影响.pdfVIP

2014 22(3) 250 ~ 256 * , , ()(HPLC) 6-BA NAA KT GA 6-BA GA NAA 3 3 KT1/2MS 0 ~ 0.2 mg L1 6-BA0.2 mg L1 NAA0.5 mg L1 KT 0.1 mg L1 GA 3 HPLC doi: 10.3969/j.issn2014.03.006 * LAI Gong-ti, LIN Yu-ling, LAI Zhong-xiong (, Fuzhou 350002, China) In order to understand the effect of different culture conditions on content of plumbagin in sundew () plantlets, the changes in plumbagin content were studied by using high performance liquid chromatography (HPLC). The results showed that the content of plumbagin in roots was higher than that in leaves. Light quality and organic element content in medium had no signifi cant differences in plumbagin accumulation in sundew plantlets, but had signifi cant effects on its growth. White light was the best for culture of sundew plantlets, followed by red light, blue light, and green light. Appropriate to reduce the organic matter content in medium could promote the growth and development of sundew plantlets. The effects of different plant growth regulator on plumbagin accumulation were in the order of 6-BA NAA KT GA3, and those on growth were in descending order: 6-BA GA3 NAA KT. Therefore, the optimum culture condition for sundew plantlets was 1/2MS as 1 1 1 basic medium under white light, supplemented with 0 0.2 mg L 6-BA, 0.2 mg L NAA, 0.5 mg L KT and 0.1 mg L1 GA . 3 ; Plumbagin; HPLC; Light; Organic matter; Plant growth regulator ()