玉米赤霉烯酮毒素降解酶基因的克隆及在毕赤酵母中的高效表达 expression of zearalenone degrading enzyme gene from gliocladium roseum in pichia pastoris gs115.pdfVIP
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玉米赤霉烯酮毒素降解酶基因的克隆及在毕赤酵母中的高效表达 expression of zearalenone degrading enzyme gene from gliocladium roseum in pichia pastoris gs115
2011年5月 中国粮油学报 V01.26,No.5
JournaloftheChineseCerealsandOilsAssociation 2011
第26卷第5期 May
1—2864/TS1530.001
DOI:CNKI:1
玉米赤霉烯酮毒素降解酶基因的克隆及在
毕赤酵母中的高效表达
刘海燕1,2 孙长坡2 伍松陵2 张艳菊1 吴子丹2
(东北农业大学1,哈尔滨150030)
(国家粮食局科学研究院2,北京100037)
摘要 以粉红黏帚霉菌株总RNA为模板,采用RT—PCR技术克隆得到玉米赤霉烯酮降解酶基因cD.
行了降解玉米赤霉烯酮能力测定。克隆得到的cDNA序列全长795bp,连续编码编码264个氨基酸;阳性克隆
子在甲醇诱导培养3—5d后,HPLC检测证实,经表达后的酶液能完全降
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