酶制剂在玉米生料发酵酒精中的应用.docVIP

JIU JIANG UNIVERSITY 毕 业 论 文 题 目 英文题目 院 系 生命科学学院 专 业 生物科学 姓 名 张振宇 年 级 06级 指导教师 李汉全 二零一零年六月 摘 要 实验所用生料发酵酒精复合酶是九江中星联生物科技公司提供的一种复合酶制剂。研究表明，其在玉米生料发酵中的合理添加有效的解决了玉米的液化和糖化问题。经过反复试验，生料酒精复合酶的最优比例为在料水比为1∶2时每200g玉米粉加01#酶（80uL）；10#酶(450uL)；A酶(200uL)；03#酶(20uL)；04#酶(5uL)；05#酶(0.4uL)；11#酶(400uL) ；23#酶(4.6uL) ；24#酶(10uL)。这使得发酵液中还原糖的含量达到酵母生长和发酵的最适浓度，避免了杂菌的大量感染。经过复合酶对淀粉的分解和转化，发酵液中残淀粉、残糊精、残还原糖、残总糖和过滤总糖的含量大大降低，最大限度的使淀粉转化为酵母菌可利用的还原糖，进而发酵转化为酒精，从而显著提高了发酵液中的酒精含量。 [关键词] 酶制剂；淀粉生料发酵；应用 Abstract The alcohol fermentation with uncooked compound enzyme is jiujiang zhongxing couplet biotechnology company provides a compound enzyme preparation. Research shows that, in the corn in the fermentation with uncooked add effectively solve the problem of corn liquefaction and glycosylated. After repeated experiments of alcohol compound enzyme raw materials for the optimal proportion than in water for 1:2 each 200g cornmeal and 01# enzymes (80uL), 10# enzyme (450uL), A enzyme (200uL), 03 # enzyme (20ul), 04# enzymes (5uL), 05 # enzyme (0.4 ul), # 11 enzyme (400uL), 23 # enzyme (4.6 ul), 24 # enzyme (10ul). This makes the result of reducing the yeast fermentation to grow and content of the optimal concentration, avoid the amount of bacteriainfection. After the decomposition of starch compound enzyme and transformation, result of residual amylum, residual dextrin and residual reducing sugar, total sugar content and filtration greatly reduced the maximum makes starch into yeast available, reducing alcohol fermentation into, thus greatly improve the result of alcohol content. [key] enzyme preparation;Fermentation with uncooked starch;application 目录 摘 要 ii Abstract iii 前 言 1 1 材料与方法 2 1.1材料 2 1.1.1供试酶制剂 2 1.1.2供试菌株 2 1.1.3原料 2 1.1.4其它仪器、材料和试剂 2 1.2方法 2 1.2.1培养基的制备 2 1.2.2酵母菌扩 4 1.2.3酒精发酵阶段 4 1.2.4发酵液试样酒精和挥发酸含量检测 4 1.2.4.1酒精含量检测 4 1.2.4.2挥发酸含量检测 5 1.2.5发酵液各组分含量