大蒜素联合长春新碱对膀胱癌BIU-87细胞增殖和凋亡的影响王允金1.DOCVIP

大蒜素联合长春新碱对膀胱癌BIU-87细胞增殖和凋亡的影响 王允金1，李永生(，穆细院1，赵 诚3，胡 钊4（350001 福建 福州，福建医科大学；协和临床医学院泌尿外科1；协和医院泌尿外科2；350004福建 福州,福建中医药大学附属人民医院泌尿外科3；361001福建 厦门,厦门中医院泌尿外科4） [摘要] 目的 探讨大蒜素联合长春新碱对膀胱癌BIU-87细胞增殖和凋亡的影响。方法 实验分为大蒜素组、 长春新碱组、大蒜素联合长春新碱组，以未用药物处理的BIU-87细胞为空白对照组。利用CCK-8法分别评价大蒜素和长春新碱对BIU-87细胞体外生长的抑制作用,通过逆转录聚合酶链反应(RT-PCR)、免疫细胞化学法和Western blot法检测各个组细胞中CDK1和CyclinB1的mRNA和蛋白表达水平的变化, 流式细胞术检测细胞凋亡情况。 结果 大蒜素和长春新碱对膀胱癌细胞的生长均有明显的抑制作用，且呈浓度依赖性；联合用药组能显著降低BIU-87细胞中CDK1和CyclinB1的mRNA和蛋白的表达，与空白对照组相比均有显著差异（P0.05）；同时能诱导细胞凋亡，联合用药组凋亡率达（54.89±0.27）%，与空白对照组、大蒜素组、长春新碱组比较有统计学意义（P0.05）。 结论 大蒜素可增强长春新碱对膀胱癌细胞增殖抑制作用，两者具有协同抗肿瘤作用；大蒜素诱导细胞凋亡的机制可能与下调CDK1和CyclinB1的表达有关。 [关键词] 膀胱癌;大蒜素;长春新碱; CDK1; CyclinB1 [中图法分类号] R737.14 [文献标志码] A Effect of allicin combined with vincristine on proliferation and apoptosis in bladder cancer cell Line BIU-87 Wang Yunjin1，Li Yongsheng2，Mu Xiyuan 1,Zhao Cheng3，Hu Zhao4 (1Department of Urology，Union Clinical College of Fujian Medical University；2Department of Urology，Union Hostpital of Fujian Medical University, Fuzhou,Fujian Province,350001；3Department of Urology，Peoples Hospital Affiliated to Fujian University of Traditional Chinese Medicine，Fuzhou,Fujian Province,350004；4Department of Urology，Xiamen Hostpital of Traditional Chinese Medicine，Xiamen，Fujian Province,361001,China） [Abstract] Objective To investigate the effect of allicin combined with vincristine on proliferation and apoptosis in bladder cancer cell Line BIU-87. Methods BIU-87 cells with different treatments were divided into an allicin group,a vincristine group,a combination group and a non-drug treatment group was the blank control group. Inhibitory effect of allicin and vincristine on bladder cancer cell Line BIU-87 proliferation was assessed by CCK-8 assay. The mRNA and protein levels of CDK1 and CyclinB1 in BIU-87 cells were detected by reverse transcription-polymerase chain reaction (RT-PCR), immunocytochemistry and Western-blot.Cell apoptosis was analyzed by flow cytometry(FCM). Results Anti-proli