枫斗类石斛cpDNApsbA-trnH的序列分析与鉴别Analysis-药学学报.PDF

药学学报 Acta Pharmaceutica Sinica 2009, 44 ( 10) : 1173 1178 ·1173 · 枫斗类石斛cpDNA p sbA-trnH 的序列分析与鉴别 邵世光, 韩 丽, 马艳红, 沈 洁, 张伟超, 丁小余* (南京师范大学生命科学学院, 江苏 南京 210046) 摘要: 本研究对常见枫斗类石斛cpDNA 的p sbA-trnH 区进行了测序, 利用MEGA 4.0 对该区序列进行了比 较分析。结果显示: 15 种常见的枫斗类石斛的p sbA-trnH 序列长度为721～767 bp, 变异位点42 个, 简约信息位 点11 个。以Kimura-2 参数计算遗传距离, 常见枫斗类石斛的种间遗传距离为0.001 3 ～0.018 3, 平均遗传距离为 0.014 8 。不同石斛种间均存在序列差异, 全序列中共出现6 处indels, 导致种间片段长度差异较大; 暂未发现所 检测的枫斗类石斛居群间在p sbA-trnH 区的序列存在差异。利用枫斗类石斛p sbA-trnH 序列数据库 遗传分析软 件, 通过对待检种的p sbA-trnH 序列的测定, 成功鉴别了待检种。cpDNA 的p sbA-trnH 区可作为枫斗类石斛分子 鉴别的候选标记。 关键词: 石斛属; 枫斗类石斛;p sbA-trnH; 分子鉴别 中图分类号: R931.5 文献标识码:A 文章编号: 0513-4870 (2009) 10-1173-06 Analysis and authentication of cpDNApsb A-trnH regions of Dendrobium species of Fengdous * SHAO Shi-guang, HAN Li, MA Yan-hong, SHEN Jie, ZHANG Wei-chao, DING Xiao-yu (College of Lif e Sciences, Nanj ing Normal University, Nanj ing 210046, China) Abstract: The p sbA-trnH regions of Dendrobium species of Fengdous were sequenced by our research group . The p sbA-trnH sequences of fifteen Dendrobium species were analyzed with software MEGA 4.0. The results showed that the lengths of sequences varied from 721 to 767 bp . The variable sites were 42 while the informative sites were 11. Genetic distances were calculated using the Kimura 2-parameter model. Genetic distances varied from 0.001 3 0.018 3 among fifteen species while the average geneti