外源调节物质对棉花幼苗耐寒生理特性的效应.pdf

棉 花 学报 Cotton Science 20 15袁27 3 冤院254―259 源调节物质对棉花幼苗耐寒生理特性的效应 1 2 1 1 1 * 慧慧 李志强 李防洲 侯振安 冶军 ( 1.石河子大学农学院 新疆 河子832003 曰2. 河子农业科学研究院 新疆石河子832003) 摘要院以棉花幼苗为试材 叶面分别喷施外源调节物质水杨酸(SA) 尧壳聚糖(CTS)尧水杨酸+ 壳聚糖(SCM) 尧水杨 酸+ 壳聚糖+ 硝酸钙渊SCCaM 冤后 在5 低温下进行处理 分别于0 尧24 h 尧48 h 及恢复处理24 h 时测定相对 电导率尧MDA 含量尧叶绿素含量 SOD尧POD尧CAT 的活性和可溶性蛋白尧可溶性糖尧脯氨酸的含量遥 结果表明院 低温胁迫前用0.7 mmol 窑L－1 水杨酸尧0.6 mmol 窑L－1 壳聚糖及复配制剂处理棉花幼苗 其叶片的SOD尧POD尧 CAT 活性和叶绿素尧 可溶性蛋白尧 可溶性糖尧 脯氨酸含量与对照相比均有不同程度的提高 相对电导率和 MDA 含量有所降低曰复配制剂处理效果优于水杨酸和壳聚糖单独施用的效果 而水杨酸尧壳聚糖尧硝酸钙三元 复配的效果比水杨酸和壳聚糖两元复配的效果好遥 表明水杨酸尧壳聚糖和硝酸钙在诱导棉花幼苗耐寒性中具 有协同效应遥 关键字院水杨酸曰壳聚糖曰棉花幼苗曰复配制剂曰耐寒性 中图分类号院S562.01 文献标志码院A 文章编号院1002-7807 20 15冤03-0254-06 10.11963/issn.1002-7807.201503009 1 2 1 1 1* Xin Huihui , Li Zhiqiang , Li Fangzhou , Hou Zhenan , Ye Jun (1. 832003, ; 2. 832003, ) We studied the effects of exogenous regulating substances on the physiological traits of cold tolerance(5 ) in cotton seedlings by spraying them with SA, CTS, SCM(SA+CTS) and SCCaM(SA+CTS+Ca), respectively. Indicators of relative con- ductivity; MDA content; chlorophyll content; SOD, POD and CAT activity; soluble protein; soluble sugar; and proline content were measured at 0 h, 24 h and 48 h. The results showed that if the cotton seedlings were treated with a compound preparation of 0.7 mmol 窑L－1 salicylic acid and 0.6 mmol 窑L－1 chitosan before stressing, then the SOD, POD and CAT activity and the solu- ble protein, soluble sugar and proline content of the seedlings improved in varying degrees compared with untreated seedlings. The effect of treating with a mixture of salicylic acid and chitosan w