中缅树鼩解偶联蛋白-1基因部分序列的扩增与分析.pdfVIP

Bioprocess 生物过程, 2014, 4, 74-81 Published Online December 2014 in Hans. /journal/bp /10.12677/bp.2014.44010 Amplification and Analysis of the Partial cDNA Sequence of UCP1 Gene in Tupaia belangeri Wenrong Gao1,2*, Wanlong Zhu2# 1 School of Energy and Environmental Science, Yunnan Normal University, Kunming 2 Key Laboratory of Ecological Adaptive Evolution and Conservation on Animals-Plants in Southwest Mountain Ecosystem of Yunnan Provinc Higher Institutes College, School of Life Science, Yunnan Normal University, Kunming # Email: gaowenrong2012@163.com, zwl_8307@163.com th th th Received: Aug. 25 , 2014; revised: Sep. 16 , 2014; accepted: Oct. 8 , 2014 Copyright © 2014 by authors and Hans Publishers Inc. This work is licensed under the Creative Commons Attribution International License (CC BY). /licenses/by/4.0/ Abstract Uncoupling protein-1 (UCP1) is a kind of heat producing protein, which distributes on the inner membrane of mitochondria. Its main function is to maintain the body’s energy metabolic balance by participating thermogenic regulation and energy metabolism in BAT. In this study, we designed primers and used reversing transcriptase polymerase chain reaction (RT-PCR). This experiment successfully obtained 444 bp BAT UCP1 cDNA core fragment of tree shrews, and we speculated that the amino acid sequence were 148 amino acids. After structure-function analysis, we found that fragment composed of these amino acid possessed 32 bp homologous sequence of UCPs and 13 bp specific sequence of UCP1 in Tupaia belangeri. After the compari