具有胆盐水解酶活力乳酸菌的筛选及16S rDNA分子生物学鉴定 Screening of bile salt hydrolase activity and application of 16Sr DNA molecular methods for identification of lactic acid bacteria.pdfVIP
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具有胆盐水解酶活力乳酸菌的筛选及16S rDNA分子生物学鉴定 Screening of bile salt hydrolase activity and application of 16Sr DNA molecular methods for identification of lactic acid bacteria
v,ww.chinadairy.net 中国乳品工业
rpgy@chinajoumal.net.cn da,/ry
6S
具有胆盐水解酶活JJ@L酸菌的筛选及1rDNA分子生物学鉴定
董改香h,王俊国2,段智变m,张和平2
育部重点实验室。呼和浩特010018)
摘要:对分离自内蒙古地区牧民家庭自制的2份酸马奶中的9椽乳酸茵,进行胆盐水解酶活力的研究。采用定性和定量两种方法,筛选
具有胆盐水解酶活力菌株,并对筛选出的茵株进行16S
生成.其余8株均没产生。游离胆酸的生成量和牛磺胆酸钠的消失量分别为0.8524mmol/L和0.8520mmol/L。即游离胆酸钠的生成量和
牛磺胆酸钠的消失量成比例。18-1-3茵株鏊定为发酵乳杆菌(Lbfermentum)。
关键词:胆盐水解酶;游离胆酸;牛磺胆酸钠;16SrDNA;乳酸茵
中图分类号:Q93-331文献标识码:A 文章编号:1001-2230(2008)11-0007-04
ofbilesalt and of16SrDNA
activity
Screening hydrolase application
methodsforidentificationoflacticacidbacteria
DoNG ZK—bianIb,ZHANG
Gai—xiangla,WANGJun—gu02,DUAN He—pin92
AnimalScienceand LifeScienceandTech—
of of
(1.ShanxiAgriculturalUniversitya.College Technology;b.CoUege
of and ofEducation
BiotechnologyEngineering,MinistryPILC,
nology,Taigu030801,China;2.KeylaboratoryDairy
Inner 010018,China)
MongofiaAgriculturalUniversity,Huhhot
Abstract:NineLact/caddbaaer/ashohtedfbm2 of home—madekoumiss Inner ofChinawereas—
traditionally produc协inMongolia
samples
sessedfortheir bilesalt salt oftheses廿赫weremeasured and
potential hydrohseactivity.Bilehydrolaseactivity byqualitativequantitative
not
methods.ILesultsshowedthat:thestrainof18—1—3 the white other8straimwere
granularmaterial,the
producedopaque
so
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