小麦秸秆发酵菌种的筛选及工艺优化 Screening and process optimization of wheat straw fermentation strains.pdfVIP

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小麦秸秆发酵菌种的筛选及工艺优化 Screening and process optimization of wheat straw fermentation strains.pdf

小麦秸秆发酵菌种的筛选及工艺优化 Screening and process optimization of wheat straw fermentation strains

201 l 3,No.1 一 商搏CERE铷ALF饲EED科IND二UST4RY 小麦秸秆发酵菌种的筛选及工艺优化 时秋月,张仲欣,任广跃,史彦珍,王雪雅,王蓝天 (河南科技大学食品与生物工程学院,河南洛阳471023) 摘 要:通过比较黑曲霉、绿色木霉、酵母茵和EM茵对小麦秸秆发酵的效果,筛选出较优的发酵菌种;对筛选出 的菌种,采用二次通用旋转组合设计进行回归试验,优化其发酵工艺参数。结果表明,4种菌种中以黑曲霉发酵效 d。在此条件下,小麦秸 果最好,通过回归试验得到的最佳工艺条件为:发酵温度29℃,接种量5%,发酵时闻3.5 秆中还原糖含量为2.13g/(100g),蛋白质含量为3.48g/(100g)。 关键词:小麦秸秆;发酵;茵种筛选;工艺优化;温度;接种量;时间 中图分类号:$816.6文献标志码:A 文章编号:1003—6202(2013)11—0039—05 and ofwheatstrawfermentationstrains Screeningprocessoptimization Shi Lantian Qiuyue,ZhangZhongxin,RenGuangyue,ShiYanzhen,WangXueya,Wang ofFoodand ofScienceand 471023,China) (College Bioengineering,HenanUniversity Technology,Luoyang fermentationstrainswerescreened thewheatstrawfermentationeffectof ABSTRACT:The optimal bycomparing Aspergillus andEMbacteria.Thescreenedstrainwas for test ro— viride,yeast designed byquadraticgeneral niger,Trichoderma regression thefermentation were resultsshowedthatthe fermenta— method,and tary processparametersoptimized.The Aspergillusniger tioneffectwasthebest thefour the conditionswereobtainedthe among strains,andopitimumtechnological by regressiontest: fermentation 29。C,inoculationamount5%,fermentationtime3.5d.Underthecondition,the con— temperature reducingsugar tentofwheatstrawwas contentwas 2.13 3.48 g/(100g),andprotein g/(i00g). KEYWORDS.-wheat amount;time straw;fermen

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