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毕赤酵母工程菌产植酸酶补料分批发酵研究 Feed-batch fermentation of Pichia pastoris engineering strain
2007年第6期
中 国 酿造
研究报告 总第171期 ·9·
毕赤酵母工程菌产植酸酶补料分批发酵研究
谢子文1,王红宁1’2木
(1.四川农业大学预防医学生物工程实验室,四川雅安625014;
2.四川大学“98522程”西南资源环境与灾害防治科技创新平台,四川I成都610065)
306.9kU/mL。
关键词:毕赤酵母:补料分批发酵:高密度发酵
中图分类号:TQ92 文献标识码:A 文章编号:2054—0571(2007)06—0009—05
Feed—batchfermentationofPichia strain
pastorisengineering
Xmzi.wenl,WANG
Hong.nin91’2木
Agricultural Innovative
(J.LaboratoryofVeterinaryMedicineandBioengineering,Sichuan
Platform ofSouthwestChina,Sichuan
of985ProjectforResourceandEnvironmentalProtectionUniversity,Chengdu
Abstract:Thefeed—batchfermentationofPichia strainPP-MS-NP“-I6was toshakeflask
eng studied.Accordingexpefi-
pastorisineering
ments,thefermentationconditionsweredefinedasfollows:theinoculumwas4%:the atgrowthandat were
optimalpH phase inducingphase
5.0and methanolconcentrationforinducementwas30 concentrationofbeanoilat andatinduc-
5.5,respectively;the g/ml;the growthphase
were DO-stat of wasthe at fermentation
1.3%and optimum
ingphase 3%,respectively.Thefeedingglycerol strategyhi曲density phase.Af-
ter for12 reached306.9kU/mlaftertheinducementofmethanolfor96h.
h,0D鲫reached145.The
feedingglycerol phytaseactivity
words:Hchia
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