水分胁迫对烤烟氨同化和生物量的影响 Effects of water stress on ammonium assimilation and biomass of flue-cured tobacco.pdfVIP

水分胁迫对烤烟氨同化和生物量的影响 梁太波，王建伟，尹启生，张艳玲，蔡宪杰，过伟民，刘阳 中国烟草总公司郑州烟草研究院，郑州高新技术产业开发区枫杨街2号450001 摘要：为明确不同水分条件下烤烟氨同化和氮素代谢差异，在盆栽条件下，研究了干旱和渍水胁迫对烤烟氨同化关键酶活性和生 同化中的作用加强；干旱胁迫严重影响烤烟植株正常生长发育，降低生物量，这可能是导致叶片氮素含量相对增加的重要原因。干 旱胁迫后复水烤烟Gs活性迅速同升，脯氨酸含最恢复到正常水平，体现了，复水对烟株的补偿效应。严莺的渍水胁迫导致烤烟植株 生理功能紊乱，氨同化关键酶活件和氮索含量均显著下降，植株生物量降低，是烤烟生产不可忽视的逆境条件。 关键词：烤烟；水分；氨同化；酶活性 doi：lO．3969／j．issn．1004-5708．2012．03．009 中图分类号：$572．07 文献标识码：A 文章编号：1004-570812012)03-0050-05 EffectsofWaterstress0nammoniumassimilationand biomassofflue·curedtobacco LIANG Tai—bo，WANGJian—wei，YINQi-sheng，ZHANGYan-ling，CAIXian．jie，GUOWei-min，LIUYang TobaccoResearchInstituteof Zhengzhou 450001，China CNTC，Zhengzhou and Abstract：Difierenceofammoniumassimilation metabolismunderdifferentwaterconditionsandhence nitrogen effectsof andwater on activitiesand werestudiedwith drought loggingkeyenzyme plantgrowth potexperiment using eultivar showedthatGSandGOGATactivitiesinleavesdecreasedandtheGS／GOGATWas Zhongyanl00．Results cycle inhibitedunder at activitiesincreased droughtvigorousgrowing NADH—GDH it amore period，while indicatingplayed roleinammoniumassimilation．TobaccoWasrestrainedand biomassdecreasedunder important plantgrowth plant wasthereasonfor contentincrease．GSactivitiesrose and contentrecoveredaf- drought，which nitrogen rapidlyproline a effectto80meextent．Water ter was