最新 苦荞麦降糖化学成分初步研究.pptVIP

* bile Kaempferol-3-O-glucoside 中文名称为“山柰酚-3-O-葡萄糖苷” 16个代谢产物和7个原型成分在粪便、尿液胆汁和血浆中的分布 No. Identification Fece Urine Bile Plasma 0-24 h 24-48h 0-24 h 24-48h 0-12h 12-24h 24-48h 0.5 h 1 h 3 h 6 h P3 Quercetin-3-O-[β-D-xyloxyl-(1→2)-α-L-rhamnoside] + + ND ND ND ND ND ND ND ND ND P5 Kaempferol-3-O-β-D-glucoside ND ND ND ND + ND ND ND ND ND ND P8 3,6-di-p-coumaroyl-1,6′-di-feruloyl sucrose + ND ND ND ND ND ND ND ND ND ND P9 1,6,6′-tri-feruloyl-3-p-coumaroyl sucrose + ND ND ND ND ND ND ND ND ND ND P10 1,3,6,6′-tetra-feruoyl sucrose + ND ND ND ND ND ND ND ND ND ND P11 N-trans-feruloyltyramine + ND + + ND ND ND ND ND ND ND M3-1 Methyl quercetin-3-O- [β-D-xyloxyl-(1→2) -α-L-rhamnoside] ND ND ND ND + ND ND ND ND ND ND M5-2 Kaempferol-glucoside monoglucuronide ND ND + ND ND ND ND ND ND ND ND M5-3 Kaempferol-diglucuronide ND ND ND ND ND ND + ND ND ND ND M5-4 Kaempferol-glucoside monoglucuronide ND ND ND ND + ND ND ND ND ND ND M5-5 Kaempferol-diglucuronide ND ND ND ND ND + + ND ND ND ND M5-6 Kaempferol-monoglucuronide ND ND ND ND + + + + + + + M7-1 Coumaric acid + + + + ND ND ND ND ND ND ND M7-2 Ferulic acid + + + + ND ND ND ND ND ND ND M9-1 Tri-feruloyl sucrose + ND ND ND ND ND ND ND ND ND ND M11-2 N-trans-feruloyltyramine glucuronide ND ND + ND + ND ND + + + + M11-4 N-trans-feruloyltyramine glucuronide sulfate ND ND ND ND + + + ND ND ND ND M11-7 N-trans-feruloyltyramine sulfate ND ND + ND ND ND ND ND ND ND ND M1 Kaempferol-glucoside monoglucuronide ND ND + ND ND ND ND ND ND ND ND M2 Quercetin-diglucuronide ND ND ND ND ND ND ND ND ND ND ND M3 Methyl quercetin glucoside monoglucuronide ND ND ND ND + ND ND ND ND ND ND M4 Quercetin-rhamnoside monoglucuronide ND ND ND ND + ND ND ND ND ND ND M5 Methyl quercetin-glucuronide ND ND ND ND + ND ND ND ND + + 小 结 通过HPLC-PDA/LTQ-FTICRMS技术，采用单体和提取物相结合的方式，进行体内代谢研究，共计发现了39个代谢产物和10个原型成分。 糖苷类化合物主要发生I相代谢的酯水解反应，黄酮类化合物主要发生I相代谢的甲基化以及 II相代谢的硫酸化和葡萄糖醛酸化反应，酰胺类主要发生II相代谢的硫酸化和葡萄糖醛酸化反应。 推测了酰胺类、黄酮类和糖苷类化合物可能的代谢途径。 大鼠原位肝肠灌流模型，能够较好进行单体化合物的代谢研究。 通过提取分离和HPLC-PD