基于聚硫堇和层层组装碳纳米管HRP的过氧化氢传感器.pdfVIP

2011 年第69 卷 化 学 学 报 Vol. 69, 2011 第18 期, 2143～2147 ACTA CHIMICA SINICA No. 18, 2143～2147 ·研究论文· 基于聚硫堇和层层组装碳纳米管/HRP 的过氧化氢传感器研究 杨绍明* 黄爱花 查文玲 魏志鹏 郑龙珍 (华东交通大学基础学院化学化工系 南昌 330013) 摘要 将电聚合和层层组装方法有效结合构筑了聚硫堇(PTH) 电子介体修饰的碳纳米管(CNTs)/辣根过氧化物酶(HRP) 多层膜无试剂H O 传感器. 利用电化学阻抗谱对CNTs/HRP 多层膜的组装过程进行监测, 用循环伏安法和计时电流法 2 2 研究了该HRP 电极的电化学行为. 探讨了酶组装层数、工作电位、pH 值和碳纳米管对电极响应的影响. 该传感器对过 氧化氢在2.0 ×10－6～6.29 ×10－3 mol/L 范围内呈良好的线性响应, 相关系数为0.998, 检测限为1.0×10－6 mol/L. 关键词 聚硫堇; 电聚合; 层层组装; 传感器; 过氧化氢 H O Biosensor Based on Poly(thionine) and Layer-by-Layer 2 2 Self-assembly of Carbon Nanotubes and HRP Yang, Shaoming* Huang, Aihua Zha, Wenling Wei, Zhipeng Zheng, Longzhen (Department of Chemistry and Chemical Engineering, School of Basic Sciences, East China Jiaotong University, Nanchang 330013) Abstract Carbon nanotubes (CNTs)/horseradish peroxidase (HRP) multilayer reagentless H O biosensor 2 2 based on poly(thionine) (PTH) mediator was fabricated by the combination of electro-polymerization and layer-by-layer self-assembly method. Electrochemical impedance spectroscopy was used to monitor the layer-by-layer self-assembly process of CNTs/HRP multilayer. The electrochemical behavior of the HRP biosensor was studied by the cyclic voltammetry (CV) and chronoamperometry (I-t). The effect of the num- ber of assembled enzyme layers, work potential, pH value and CNTs were