结缔组织生长因子在慢性阻塞性肺疾病血管重建中表达(英文).pdfVIP

1663 现代生物医学进展 Progress in Modern Biomedicine Vol.11 NO.9 MAY.2011 · · Expression of Connective Tissue Growth Factor in Pulmonary Vascular Remodeling with Chronic Obstructive Pulmonary Disease LIU Xian -xian, ZHANG Jie, CUI Shi-chao, YU Wen -cheng△ (Dep artment of Resp irology, The Affiliated Hosp ital of Qingdao University Medical College, Qingdao Shandong 266003) ABSTRACT Obj ective: To investigate the expression and significance of connective tissue growth factor (CTGF) in pulmonary vas- cular remodeling in patient with chronic obstructive pulmonary disease (COPD). Method: According to their lung function, 30 male lung carcinoma patients, who had smoking history and operation in thoracic surgery, were divided into 2 groups: control group (patients with normal lung function) and COPD stable group (patients with COPD), with 15 patients in each group. The lung specimens were obtained from adjacent noncancerous tissue. Morphmetric characteristic of the pulmonary vascular remodeling was observed by HE and Masson trichrome staining. The expression of CTGF and PCNA proteins in pulmonary muscular arteries were evaluated by immunohistochem- istry methods. Results: (1) The expression of CTGF, PCNA, artery wall area / total area (WA%) and collagen thickness were increased significantly in pulmonary arteries of COPD group compared with those of the control group. (2) The expression of CTGF in smooth muscle cells was correlated with the thickness of the vessel wall, collagen and the expression of PCNA r= 0.81, 0.68, 0.86, P ＜0.05).