利用DH群体构建烤烟分子标记遗传连锁图-中国烟草学报.PDFVIP

　 DH 35 DH 1 1 1 1, 2 1 3 肖炳光 　徐照丽 　陈学军 　申爱荣 　李永平 　朱　军 [1 (), 　　653100;2 　　6502013; 3 　　310029] 　:Speight G-28 NC2326 137 DH , ISSR RAPD , 27 、 10 ISSR 147 RAPD 。 1838.2cM, 14.1cM。22, 10 LG9 , 。 , 。 :;ISSR;RAPD; :S572　　　:A　　　:1004-5708(2006)04-0035-06 Genet ic linkage map constructed by using a DH population for the flue-cured tobacco 1 1 1 1,2 1 3 XIAO ing-guang 　XU Zhao-li 　CHEN Xue-jun 　SHEN Ai-rong 　LI Yong-ping 　ZHU Jun [1China Tobacco reeding (Southern)Center, Yunnan Institute of Tobacco Science, Yuxi　653100; 2 College of Plant Protection, Yunnan Agricultural University, Kunming　6502013; 3 Institute of ioinformatics, Zhejiang University, Hangzhou 　310029] Abstract :A molecular genetic map of flue-cured tobacco was constructed with a 137 doubled haploid (DH)population from a cross of 2 flue-cured tobacco varieties Speight G-28 and NC2326 by using ISSR and RAPD as markers.The map consisted of 27 linkage groups which included 10 ISSR and 147 RAPDmarkers, and covered 1838.2 cM with an average distance of 14.1 cM.Among the 22 distortedmarkersdistributed in the map, 10 markerswere mapped on the linkage group LG9, and the oth- erswere mapped on diverse linkage groups respectively.It is the first molecular genetic linkage map of flue-cured tobacco in theworld and is fundamental to the genetic mapping of important traits and marker-assisted selection for flue-cured tobacco. Key words:flue-cured tobacco;ISSR;RAPD;genetic linkage map 　　 DNA N .p lumbaginif olia ×N .long if lora 99F2 , , ;Nishi [4] ,