巨噬细胞移动抑制因子表达与复发性自然流产关系.pdfVIP

47 5 V ol. 47, N o. 5 20 11 10 A CT A A CA DEM IA E M ED ICIN AE Q IN G DA O U N IV ERS IT A T IS O ct ober 20 11 巨噬细胞移动抑制因子表达与复发性自然流产关系 , , , , ( , 266003) [ ( M IF) ( RSA ) ( SP) , 35 RSA 45 ( ) 蛻膜组织中M IF 的定位与蛋白表达, 采用R TPCR 技 蛻膜组织M IF m RN A 表达水平, 采用酶联免疫吸附试验( EL ISA ) 检测血清 M IF 水平 M IF , RSA M IF , RSA M IF M IF m RN A , ( t= 7. 0 89~ 40. 610, P 0 . 01) RSA M IF , ( t= 8. 903, P 0. 0 1) M IF 蛻膜组织中的M I F m R N A 表达水平呈 相关( r = 0. 87 3 0. 657, P 0 . 0 1) ; M IF ( r= 0. 7940. 616, P 0 . 0 1) M IF m RN A RSA RSA , M IF RSA [ , ; ; ; [ R714. 25 [ A [ 20 11) THE RELATIONSHIP BETWEEN MACROPHA GE MIGRATION INHIBITORY FA CTOR EXPRESSION AND RECURRENT SPON TANEOUS ABORTION S UN S UMEI , LI U F URON G, ZH A N YIN G, YE YUANH UA , ZH A N G N I N G ( D epart ment of Obs t et rics , T he A f fil iat ed H ospit al of Q ingdao U niversit y M edical College, Q ingdao 266003, China) [ ABSTRACT] Obj ecti ve T o in vest igat e t he ex pres sion of macrophage mig rat ion inh ibit ory fact or ( M IF) in seru m and villi in early pregnancy and t h eir as sociati on w it h recurrent s pon taneous abort ion ( RS A ) . Methods A t otal of 35 RS A pat ient s ( RS A g rou p) and 45 n ormal early pregn ant w omen un dergoing art if icial abort ion ( con t rol group) were recruit ed. T h e expressions of M IF m RN A and M IF prot ein in villi and d eciduas were det ermined by SP m et hod; t he M IF mR N A expression in villi and d eciduas by re verse t ranscriptasepol ymerase chain react ion ( RTP CR) ; t he serum M IF levels w ere det ect ed by using