巨噬细胞移动抑制因子在不明原因早期复发性流产者血清_绒毛和蜕膜中的表达及意义.pdfVIP

2010 3 1 4 张 钰 李俊霞 顾 艳 李 奕 : (M IF) ( UERSA) : 2008 1~ 12 30 ( UERSA ) 30 ( ) , ELISA M IF, RT - PCR M IF- mRNA, : UERSA M IF ( 10. 98 3. 02ng /m l) ( 1 . 53 5. 34ng/m l), M IF - mRNA 2- CT ( 0. 022 0. 001; 0. 9 0. 58) ( 1. 290 1110; 1. 20 0. 85), M IF M IF- mRNA ( r= 0. 69, 0 1): M IF, Expression ofM acrophageM igration Inhibitory Factor in Serum, Villi and Deciduas in W omen with Unexplained Early ecurrent SpontaneousAbortion Zhang Yu, LiJunxia, Gu Yan, et al. Departmentof f amilyplanning, The Second H osp ital of Tianjin edicalUniversity, Tianjin 300211 Abstract Objective: To exp lore the expression ofm acrophage m igration inh ibitory factor(M IF) in serum, villi and deciduas inw om enw ith norm al early pregnancy and unexp lained early recurrent spontaneous abortion, and the role ofM IF contributing to the occurrence of unexplained early recurrent spontaneous abortion. M ethods: Th irty wom en w ith unexplained early recur rent spontaneous abortion ( the UERSA group) and 30 w om enw ith norm al early pregnancy ( the norm al pregnant group) were recruited in the SecondH osp ital of T ianjinM edicalUn iversity bewt een January 2008 to Decem ber 2008. The serum M IF levels w ere assayed using enzym e- linked mi munosorbent assay ( EL ISA) k its, and the expression ofM IFmRNA in the villi and de ciduas were determ ined by real- tmi e fluorescence quan titative polym erase chain reaction. Results: The serum M IF level in the UERSA group ( 10. 98 3. 02ng /m l) w as sign ifican t low er than that of the norm al pregnant group ( 1 . 53 5. 34ng/m l). The expression ofM IF mRNA in the villi and deciduas in theUERSA group ( 0. 022 0. 001; 0. 9 0. 58) w ere sign ificant low er than those of the norm al pregnant group ( 1. 290 1. 110; 1. 20 0. 85). The serum M IF level had a po