铁皮石斛对高血压左室肥厚的病理研究.docVIP

铁皮石斛对高血压左室肥厚的病理研究 　　【摘要】目的探?铁皮石斛对自发性高血压大鼠血压、左室重量指数、心肌组织病理改变及在左室肥厚中所起的作用。 　　方法采用自发性高血压大鼠（SHR）和正常血压对照组大鼠（WKY）作为研究对象，将32只12周龄SHR随机分成实验对照组（SD组）、铁皮石斛低剂量组（SL组）、铁皮石斛中剂量组（SM组）、铁皮石斛高剂量组（SH组），每组8只，另8只12周龄WKY为空白对照组，采用尾动脉血压仪测量大鼠的收缩压和舒张压，连续药物干预6周后，处死大鼠，称体重、心脏重量和左室重量，计算出心脏指数（CI）和左室重量指数（LVMI），取心肌组织做病理HE染色，观察大鼠心肌细胞的病理改变。 　　结果与对照组比较，第12周、14周及16周SHR大鼠收缩压和舒张压明显下降（P0.05），第16周SD组、SL组与SH组间的大鼠CI和LVMI比较，差异有统计学意义（P0.05），各治疗组CI、LVMI、心肌细胞直径、周长及面积随剂量增加而减小（P0.05）。 　　结论铁皮石斛治疗高血压效果显著，能有效控制血压水平，扭转心室重构等病理改变。 　　【关键词】铁皮石斛；高血压；左室肥厚 　　中图分类号：R544.1文献标识码：ADOI：10.3969/j.issn2017.01.013 　　An pathological study of dendrobium candidum on left ventricular hypertrophy 　　[HJ1*2][HJ] 　　WANG Junbao1，PAN Xingshou2，HUANG Zhaohe2 　　（1. Graduate School， 2.Cardiovascular Department of Affiliated Hospital， Youjiang Medical University for Nationalities， Baise 533000 ） 　　[HJ2][HJ] 　　【Abstract】ObjectiveTo explore influence of dendrobium candidum on blood pressure， left ventricular weight index， pathological changes of myocardial tissue of spontaneously hypertensive rat as well as on left ventricular hypertrophy. 　　MethodsSpontaneously hypertensive rats （SHR） and normotensive control rats （WKY） were selected as research objects. Thirtytwo 12weekold SHR were randomly divided into experimental control group （SD group）， low dose dendrobium candidum group （SL group）， middle dose dendrobium candidum group （SM group） and high dose dendrobium candidum group （SH group）， with 8 rats in each group. And the other eight 12weekold WKY were selected as blank control group. Systolic blood pressure and diastolic blood pressure were measured by tail artery blood pressure apparatus. After 6 weeks of continuous drug intervention， rats were sacrificed， and weight， heart weight and left ventricular mass as well as cardiac index （CI） and left ventricular mass index （LVMI） were calculated. In addition， myocardial tissue was taken for pathological HE staining， pathological changes of myocardial cells in rats were observed.