山楂中总黄酮和多糖的提取工艺研究.pdfVIP

30 9 Vo.l 30 No. 9 2010 9 Journal of CentralSouthUniversity of Forestry Technology Sep. 2010 1 1 2 1, 2 , , , ( 1. 中南林业科技大学 森林植物实验教学示范中心, 湖南长沙 410004; 2. 湖南中医药大学 药学院, 湖南长沙 410208) : ,, :3, 1412 12, 60 min, 3. 02. 52. 5 h , : ; ; ; ; ; ; : Q 946 : A : 1673 923X( 2010)09 0162 04 Extracting conditionsof total flavones and polysaccharide from Crataegus p innatif ida 1 1 2 1, 2 LIU Q iaoli, WANG L iuyun , YAN Jianye, WANG Yuanqing ( 1. Demonstration Center forTeaching Expermi ent of Forest Plant, CentralSouthUniversity of Forestry andTechnology, Changsha 410004, Hunan, China; 2. School of Pharmacy, HunanUniversity ofTraditional ChineseM edicine, Changsha 410208, Hunan, China) Abstract: The polysaccharide amount and total flavones amount of Crataeg us p innatif ida ere chosen as the assessment in dex, and some factors such as extraction tmi es, macerating tmi e, extracting tmi e, solvent volume etc ere adopted, the opti mum extraction process as selected based on single factor analysis and ith the uniform design. The optmi al process condi tions ere: macerated for 60 min in ater, extracting for 3 tmi es, ith ater of 14, 12, 12 tmi es of the drugm ixture respective ly, extracting tmi e of 30 hours, 2. 5 hours, 2. 5 hours, respectively. The extraction process of optmi ization can almost extract all the effective compo