基于CDDP标记铁皮石斛抗性种质筛选与多样性研究.docVIP

基于CDDP标记铁皮石斛抗性种质筛选与多样性研究 吴永辉 张君毅 司灿 林清郁 华侨大学生物工程与技术系 X 关注成功！ 加关注后您将方便地在 我的关注中得到本文献的被引频次变化的通知！ 新浪微博 腾讯微博 人人网 开心网 豆瓣网 网易微博 摘????要： 目的 利用CDDP标记技术对43份铁皮石斛Dendrobium officinale野生和栽培种质进行初步抗性筛选和遗传多样性分析, 为铁皮石斛产业以及优良品种筛选保护提供理论基础。方法 从21条CDDP引物中筛选出产物清晰、多态性好的引物对供试材料基因组DNA进行扩增。结果 16条引物共扩增出了151条带, 其中多态性条带144条, 多态性比率95.7%, 表明供试材料具有丰富的遗传多样性。种群遗传多样性结果表明, 3个种群多态性位点比例 (PPL) 为65.56%82.12%, 种群间基因分化系数 (Gst) 为0.110 2, 基因流 (Nm) 为4.035 4, 说明人为选择亦可能促进铁皮石斛的遗传多样性。结论 铁皮石斛人工栽培种具有丰富的遗传多样性, 同时统计结果分析表明, 43份材料中可以初步筛选出8份具有潜在优良抗性的植株。 关键词： 铁皮石斛; CDDP标记; 遗传多样性分析; 抗性筛选; 多态性位点比例; 作者简介：吴永辉 (1991—) , 男, 在读硕士, 研究方向为药用植物资源开发与利用。Tel-mail:qqanqq@126.com 作者简介：张君毅, 副教授, 硕士生导师, 主要研究方向为药用资源开发与利用。Tel-mail:junyi@ 收稿日期：2017-06-28 基金：福建省引导性项目:铁皮石斛良种选育与关键栽培新技术研究 (2015N0028) Screening and diversity of resistance germplasm of Dendrobium officinale by CDDP markers WU Yong-hui ZHANG Jun-yi SI Can LIN Qing-yu Department of Bioengineering Biotechnology, Huaqiao University; Abstract： Objective Conserved DNA-Derived Polymorphism (CDDP) markers were used in the study of the genetic diversity of 43 Dendrobium officinale and preliminary resistance screening, in order to provide a theoretical basis for the screening of D. officinale and the selection of fine varieties. Methods A total of 21 CDDP primers were used to amplify the genomic DNA of the test material with clear and polymorphic primers. Results Sixteen primers generated 151 bands, of which 144 (95.7%) were polymorphic. The results of data analysis on three population showed that the percentage of polymorphic locus (PPL) were between 65.56% and 82.12%, coefficient of genetic differentiation among natural populations (Gst) was 0.110 2, and the total gene flow (Nm) was 4.035 4. Indicating that anthropogenic factors may also promote the genetic diversity of D. officinale. Conclusion The genetic diversity of D. officinale was rich, and the results showed that there were eight plants with potentially good resistance among 43 materials.