miR-137抑制人葡萄膜黑素瘤细胞的增殖和迁移.docVIP

精品论文 参考文献 miR-137抑制人葡萄膜黑素瘤细胞的增殖和迁移 　郑娜芬1 林俊汕2 郭佳琳2 叶梓莹2 　　（1中山大学孙逸仙纪念医院病理科 广东广州 510288） 　　（2中山大学孙逸仙纪念医院增城院区 广东增城 511300） 　　【摘要】目的:探讨人眼葡萄膜黑素瘤细胞系M23转染miR-137后对细胞增殖和迁移能力的影响及其分子机制的初探。方法:采用阳离子脂质体LipofectamineTM2000将miR-137mimics转染M23细胞，应用CCK-8法检测转染后细胞增殖变化，划痕实验检测细胞迁移能力变化。Westernblot检测PTEN、总AKT和磷酸化AKT蛋白表达。结果:转染miR-137后，实验组的细胞增殖较对照组明显减低（t=-18.754，P=0.000；）；在36h和72h时间点，实验组细胞的划痕愈合程度均明显低于对照；实验组的PTEN蛋白表达增加，p-AKT蛋白减低，AKT蛋白表达无明显变化。结论:miR-137通过上调PTEN蛋白表达和减低AKT磷酸化活性从而抑制人眼葡萄膜黑素瘤细胞的增殖和迁移能力。 　　【关键词】 葡萄膜黑素瘤 miR-137 增殖 迁移 　　【中图分类号】R73 【文献标识码】A 【文章编号】2095-1752（2014）20-0130-03 　　miRNA-137 suppresses human uveal melanoma cell line proliferation and migration 　　ZHENG Nafeng, LIN Junshan, GUO Jialing, YE Ziying (Department of Pathology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, China) 　　【Abstract】 Objective To study the effects of miR-137 on proliferation and migration in human uveal malignant melanoma cell lines M23 and investigate its action mechanisms.Methods miR-137 mimics were transfected M23 by LipofectamineTM 2000. The change of cell proliferation and migration of M23 cells were studied by Cell Counting Kit-8 (CCK-8) assay and wound migration assay, respectively. PTEN, p-AKT and AKT proteins expression were investigated by western blot. Results miR-137 significantly inhibited cell proliferation and migration. PTEN protein was markedly increased and the phosphorylation of AKT was markedly reduced by miR-137 mimics treatment, respectively, but miR-137 was no effect on unphosphorylated AKT. Conclusion miR-137 exhibits inhibitory effects on proliferation and migration in human uveal melanoma cell through up-regulated PTEN protein expression and inhibit the phosphorylation of AKT 　　【Key words】 uveal melanoma miR-137 proliferation migration 　　葡萄膜黑素瘤是成人中最常见的眼内恶性肿瘤，具有易侵袭和转移的特点[1,2]。虽然眼葡萄膜黑素瘤和恶性黑素瘤细胞都来源于色素细胞，但其发生发展的分子机制却不像恶性黑素瘤那么明了[3]。研究显示葡萄膜黑素瘤死亡率很高，主要是因为该肿瘤具有极高的血行转移概率，其中肝转移占了全部转移的9