参乌胶囊及其有效成分二苯乙烯苷对老年大鼠海马-中国试验动物学报.docVIP

参乌胶囊及其有效成分二苯乙烯苷对老年大鼠海马区神经生长因子及其受体表达的影响 王蓉1,2，蔡彦宁3，赵志炜2，唐玉4，李林1* (1.首都医科大学宣武医院药物研究室；2.中心实验室； 3.神经生物研究室，北京100053；4. 首都医科大学基础医学院药理[摘要] 目的：观察参乌胶囊二苯乙烯苷对老年大鼠海马(NGF)及其受体表达的影响。方法：老年SD大鼠从21月龄始，分别灌胃给予0.8g?kg-1、1.6g?kg-1）和TSG低、高剂量（0.03g?kg-1、0.06g?kg-1）至24月龄。海马，免疫组织化学染色海马。NGF mRNA和TrkA mRNA水平；SW和TSG对NGF蛋白表达有明显的增高作用，高剂量SW和TSG对TrkA表达的上调作用显著。结论：SW和TSG能够增高老年大鼠海马区NGF及其受体水平，提示它们可作用在神经元信号转导通路的起始端，有利于提高神经元的存活和功能。 [关键词] 参乌胶囊二苯乙烯苷TrkA；海马；老化 [中图分类号] R-332，R338.1+3 [文章编号] Effects of Shenwu capsule and tetrahydroxystilbene glucoside on expression of NGF and its receptor in hippocampus of aged rats WANG Rong1,2,, CAI Yan-ning3, ZHAO Zhi-wei2, TANG Yu4, LI Lin1* (1. Department of Pharmacology; 2. Central Laboratory; 3. Department of Neurobiology, Xuanwu Hospital of Capital Medical University, Beijing 100053, China; 4. Department of Pharmacology, Capital Medical University, Beijing 100069, China) [Abstract] Objective To investigate the effects of Shenwu capsule (SW), a new combination drug of traditional Chinese medicine, and its component tetrahydroxystilbene glucoside (TSG) on the expression of never growth factor (NGF) and its receptor in the hippocampus of aged rats. Methods The aged rats were intragastrically administrated by SW (0.8g?kg-1 and 1.6g?kg-1) and TSG (0.03g?kg-1 and 0.06g?kg-1) for 3 months (from 21 to 24 months of age), respectively. Real-time PCR was applied to measure NGF mRNA and TrkA mRNA in the hippocampus. Immunohistochemical staining and Western blot methods were used for detecting the expression of NGF and TrkA in the hippocampus. Results From the levels of gene and protein, the mRNA and protein expression of NGF and TrkA showed a decrease in the hippocampus of aged rats. Intragastrical administration of SW to the aged rats up-regulated the expression of NGF mRNA and TrkA mRNA in the hippocampus; SW and TSG enhanced the expression of NGF, and the high dose of SW and TSG elevated the expression of TrkA. Conclusion SW and TSG increased the expression of NGF and it