左归丸对化疗致pof模型小鼠卵巢cx37蛋白-中药新药与临床药理.docVIP

左归丸对化疗致POF模型小鼠卵巢缝隙连接蛋白Cx37及mRNA表达的影响 阳松威1，孙晓峰2，贺又舜2，陈 聪2，龚力民1（1. 湖南中医药大学药学院，湖南 长沙 410208；2.湖南中医药大学中医学院，湖南 长沙 410208） 摘要：目的 通过观察左归丸对化疗致卵巢早衰（POF）模型小鼠卵巢Cx37蛋白、mRNA表达的影响，探讨左归丸治疗POF的作用与机制。方法 将昆明雌性小鼠72只随机分为空白组、模型组、戊酸雌二醇片组、左归丸低、中、高剂量组。除空白组小鼠腹腔注射等量生理盐水外，其余各组小鼠均按30 mg·kg-1剂量腹腔注射环磷酰胺，每天1次，连续注射20 d，复制POF模型；同时从造模第1天开始按剂量要求进行灌胃给药，每天1次，连续给药30天。末次给药2 h后，摘眼球法处死小鼠，随即剖开腹腔，摘取双侧卵巢，剔除周围附着的脂肪组织，一部分卵巢组织液氮保存，采用RT-PCR法检测卵巢Cx37 mRNA表达；一部分卵巢组织进行匀浆后采用western-blot 法检测卵巢Cx37蛋白的表达；一部分卵巢以4%多聚甲醛溶液固定，常规石蜡包埋，切片后采用免疫组化方法检测卵巢组织Cx37蛋白的分布。结果 左归丸可有效增强化疗致POF模型小鼠卵巢Cx37mRNA基因表达（P0.05，P0.01），增强卵巢组织Cx37蛋白表达（P0.05），增加Cx37蛋白在卵巢颗粒细胞中的分布（P0.05）。结论 左归丸可有效增强化疗致POF模型小鼠卵巢组织Cx37mRNA表达，上调Cx37蛋白在卵巢组织中的分布与表达，改善化疗致POF模型小鼠卵巢组织缝隙连接功能，恢复卵巢功能。 关键词：卵巢早衰（POF）；左归丸；Cx37；缝隙连接；mRNA；蛋白；机制 中图分类号285.5 文献标码A 文章编号1003-9783（20）0- doi：10.3969/j.issn.1003-9783.2016.01.0 Effect of ZuoGuiWan on the expression of ovarian gap junction protein Cx37 and mRNA of chemotherapy-induced POF mice model YANG Songwei1, SUN Xiao feng2*,HE You-Shun2, CHENG Cong2, GONG Li-Ming1 College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208 Hunan, China; 2.College of Traditional Chinese Medical, Hunan University of Chinese Medicine, Changsha 410208 Hunan, China.) Abstract objective By observing the effect of ZuoGuiWan on the expression of ovarian gap junction protein Cx37 and mRNA of chemotherapy-induced POF mice model,and to explore the mechanism.Methods 72 KM female mice were randomly divided into control group,model group,estradiol valerate tables group,ZuoGuiWan low,medium and high dose groups.In addition to the control group of mice were injected with saline,the remaining mice in each group wrer intraperitoneal injection of CTX,once a day for consecutive 20 days to copy POF model.At the same time from the first day of modeling, the mice were administered orally according to the dosage requirements,once a day for consecutive 30 days.2 h after the last administration,mice were sacrificed by taken the eyes,the cut open the abdomina