芒果老叶在增强 uv-b 辐射处理下的损伤和保护反应.pdfVIP

植物生理学通讯 第46 卷第8 期, 2010 年8 月 787 芒果老叶在增强UV-B 辐射处理下的损伤和保护反应 * 刘鹏, 周开兵 , 潘学锋 海南大学热带作物种质资源保护与开发利用教育部重点实验室, 海口 570228 提要: 以‘ 台农一号’芒果盆栽苗离体老叶为试材, 研究增强UV-B 辐射条件下芒果老叶的损伤和保护反应。结果表明: UV- B 辐射处理使芒果叶片MDA含量和相对电导率升高、叶绿素含量和叶绿素a/b 降低, 表明叶片受到损伤, 且随处理时间延 长叶片损伤加重。UV-B 辐射处理叶片可溶性蛋白含量、抗氧化酶(SOD 、CAT 、POD)活性、保护色素(类胡萝卜素、类黄 酮)和还原型GSH含量显著高于对照叶片, UV-B辐射处理叶片维生素C含量显著低于对照叶片, 表明增强UV-B 辐射可诱 导叶片细胞通过提高活性氧清除能力和积累保护色素而直接吸收部分UV-B辐射来提高抗增强UV-B辐射损伤的能力。 关键词: 增强UV-B 辐射; 芒果; 老叶; 损伤; 保护反应 The Damages and the Protection Response of Old Leaves of Mango (Mangifera indica L.) under Enhanced UV-B Radiation * LIU Peng , ZHOU Kai-Bing , PAN Xue-Feng Key Laboratory of Protection and Development Utilization of Tropical Crop Germplasm Resources, Ministry of Education, Hainan University, Haikou 570228, China Abstract: Old leaves of mango potted young trees were used to study damages and protection response under the enhanced UV-B radiation. The results showed that after UV-B treatment the content of MDA and the relative conductivity were higher, while the content of chlorophyll and the ratio of chlorophyll a/b became lower. It indicated the enhanced UV-B radiation caused the damages of leaves, which would be worse with longer treat- ment period. The UV-B treated leaves had higher contents of soluble protein, protective pigments (flavonodis, carotenoids) and reducd GSH, higher activities of antioxidant enzymes (SOD, CAT and POD), and lower con- tent of vitamin C than CK. It indicated the enhanced UV-B radiation induced elevated resistance of enhanced UV- B radiation in cells through intensifying the oxidant scavenging activities and absorbing part of the enhanced UV- B radiation. Key words: enhanced UV-B radiation; mango (Mangifera indica); old leaf; damage; protection response 太阳辐射中的紫外线波长介于200~400 nm, 分 芒果在我国号称“ 热带水果之王”, 海南是我 为C 区(200~280 nm) 、B 区(280~320 nm)和A 区 国最大的芒果产区