A Comprehensive Bioinformatics Analysis of the Lipoxygenases Superfamily in Shewanella Woodyi Strain (Strain ATCC 519081MS32).pdfVIP
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JournalofMaterialsScienceandEngineeringB4(2)(2014)34-41
国 LI墨 HIN6
A ComprehensiveBioinformaticsAnalysisofthe
LipoxygenaSeSSuperfamilyinShewanellaWoodyi
Strain(StrainATCC519081MS32)
SilvanaPomp6iaVal—MoraesandElianaGertrudesdeM acedoLemos
Univers砂 ofEstadualPaulistaUNESP/FCA DepartmentTechnology,LBMP,ViadeAcessoProf.PauloDonatoCastellane,s/n.
Jaboticaba1.saoPauloBrazil
Received:January05,2014 /Accepted:Jnauary31,2014/Published:February25,2014
Abstract:Sbewna ellabacteriahaverealpotentialforthebioremediationofcontaminatedenvironmentalandpresentsadifferential
thatarethreelipoxygenasesdescribedinitsgenome.Lipoxygenases(LOX)areafamilyofiron-containingenzymesthatcatalyzethe
dioxygenationofpolyunsaturatedfattyacidsinlipids.Theyoccurubiquitouslyinplantsandmammals,na donlyrecently,theyhave
beendetectedincoral,moss,fungiandanumberofbacteriaaswel1.Inthiswork,analyzedthreeenzymeslipoxigenasesdescribed
forShewanellawooay depositedinGeneBankasprobableLOX geneintheSwoo 2318 woodyfATCC 51908:ProteinsCode
一
(ACA86597.1)hypotheticalprotein,(ACA87192.1)arachidonatel5-lipoxygenasenad(AEF01209.1)arachidonate15-lipoxygenase
precursorthestrain woodyiDSM 12036.Thebioinofrmaticstoolstendtosolveimpossibleproblemstobeaddressedinthepast
decades.Theseanalysesidentifiedwerethethreeproteinsdescribed as1ipoxygenaseshavedifferentsecondary structures.the
phosphorylationsites;theproteinGRAVY (grandaverageofhydropathy)andproteinisoelectricpointaredistinct.However,binding
siteisFeofrthreelipoxygenases.Thissuggestedthatthreelipoxygenasesdeservespecialattentionofrworkinsitu,duetopeculiar
characteristicsnadstillnotknow whatistheneedofS.woodyfhaveinitsgenomethreegenesencodingthesameprotein.
Keywords:Arachidonate,crystallography,hypotheticalprotein
1.Introduction
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