鸡IL2基因的克隆及GSTchIL2融合蛋白的表达.pdfVIP

ofthe10th of AssociationofAnimaland Branch,Chinese ProceedingsSymposiumBiological VeterinaryScien—c—e Constructionof DNA forRiemerella library genomic anatipestifer Xiuhua2 QiDongmei7、2、3，QinZhonghua2，XieMingquan2，LinXuye7，CaiJianpingz，WangXin92、3，Ye G．D．Winsun Pharmaceutical 2Institute (J CO．，Ltd 510630； Biology Guangzhou of Veterinary Sciences 510640； Me函cine，G．D．AcademyofAgriculturalGuangzhou 3．SouthChina Medicine 510640) AgriculturalUniversityVeterinary collegeGuangzhou AbstractRiemerella infectionis themost infectiousdiseaseoffarm anatipestifer probably economicallyimportant ducksworldwide．Inthis DNAofriemerella 1was San3AIand study．thegenomic anatipestiferserotypedigested而tll 0．07—4kb wererecovered．ThebacterialDNA was tothelinearPUCl8andusedto fragments digested fragmentsligated transform Escherichiacoli DNA forRiemerella 1sue— JMl09，constructed competent genomiclibrary anatipestiferserotype of about numberbacterialrecombinantsobtainedwas 20000．This canofferanefficientnew ceedly．The genomic library forriemerella functional platformtechnology anatipestifergenomics． wordsRiemerella DNA Key