鸡IL2基因克隆及其重组质粒的构建与表达.pdfVIP

主国堂皇塑堂垒璺垦堕垒二塑专业委员会2003年学术年会 ·l65· and ofNP ofAvian CloningSequencinggene InfluenzaVirusH9N2 isolatedinHubeiand subtype Its InE．coli Expression HuSi shun，LiZi一1i，XiaoYun—cai，ShiDe—shi， Xu Gui--zhi．Biren’ Qing-rong，ChengFeng，Wang Ding ofVeternary College Medicine，HuazhongAgricultural 430070．【1^… University．Wuhan Abstract：Thefull cDNAof from Avian length H9N2 of nucleoprotein(NP)genesubtype Virus(AIV)，isolatedinHubei，waspurifiedwiththemethodofthe chain reaction(RT—PCR)andclonedinto Tvectortobe aresult pMDl8 1542 was sequenced．As bp indicatedNPis conservativeand NP identified．Homologyanalysis very stable．Additionally．the was cloned intoan constructtherecombinant directionally expressionvector，pGEX——KG．to plasmid identifiedwith restriction NPwastransformedintoBL2lcondonto pKG—NP．After enzyme，pKG plus IPTG，a fusion was express．Inducedby expressionprotein--GST——NP high and showedthatGST——NPwasmeasuredabout84．000 to analysisWestern——blotting specific AIV．Thisindicatedthatthe is in and be to