鸭瘟毒单克隆抗体的制备及鉴定.pdfVIP

其它病毒病 Isolationand IdentificationofDuckEnteritis Virus Honghai Wan91，XueyingHu2，Jingliang Sul，ShuanghuiHel，YongyouCheng 1CollegeofVeterinaryMedicine，China AgriculturalUnlversity．Beijing。100094 2College∥AnimalHusbandry枷ef…n7y U…ive h Medicine，HuazhongAgriculturality…Wn，430070 AbstractTwostrainsof duckenteritis viruses SDand wild—type desiganatedBJwereisolatedfrom commercial duckflocksin and meat—type Shandong duck Beijingusing embryo Both strains effects48hours cytopathic indirect cause．d post一一inoculation．Animmunofluorescent basedonmonoclonal for antibody(MeAb)wasdevelopeddetectionofDEV ininfectedDEF．The antigen fluorescentseenincell weremost about48h profiles spots significant infectedwithSDstrain experimentally showed of100 with elinical mortality percent and typical signs characteristiclesionsofduckvirusenteritis．DEVwere detectedinthe andbursaof antigens 1iver，panereas infected MeAb based experimentallyducklingsby immnoperoxidase