云南龙竹的快速繁殖和离体保存研究.PDFVIP

2012,41(2):27-31. Subtropical Plant Science 云南龙竹的快速繁殖和离体保存研究 1,2 2 2 1 李春芳 ，程治英 ，杨俊波 ，熊 智 （1.西南林业大学 林学院，云南 昆明650224；2. 中国科学院 昆明植物研究所，西南野生生物种质资源库，云南 昆明 650204 ） 摘 要：本研究以干冷保存（-20 ℃，相对湿度 15% ）150 d 的云南龙竹（Dendrocalamus yunnanicus ）颖果为 实验材料，通过种子萌发建成丛芽无菌无性系进行快繁和离体保存。研究表明，丛芽增殖的最佳培养基为MS + 6-BA 2 mg/L + NAA 0.2 mg/L ，蔗糖浓度以3%为佳；生根培养的最佳培养基为 MS + IBA 1 mg/L + NAA 1 mg/L ， 以单芽诱导生根为好；离体保存丛芽的最适培养基为MS + 6-BA 0.5 mg/L + NAA 0.2 mg/L；当培养温度由室温 (25 ±3)℃降低至(20 ±3)℃和 12 ℃时，其继代周期可由原来的2 个月分别延长至 4 个月和 8 个月。在组织培养 过程中发现白化苗或叶色变异现象。 关键词：云南龙竹；丛芽；快速繁殖；离体保存 Doi: 10.3969/j.issn.1009-7791.2012.02.007 中图分类号：Q943.1 文献标识码：A 文章编号：1009-7791(2012)02-0027-05 Rapid Propagation and In vitro Conservation of Dendrocalamus yunnanicus 1,2 2 2 1 LI Chun-fang , CHEN Zhi-ying , YANG Jun-bo , XIONG Zhi (1.College of Forestry, Southwest Forestry University, Kunming 650224, Yunnan China; 2.Kunming Institute of Botany, Chinese Academy of Sciences, The Germplasm Bank of Wild Species, Kunming 650204, Yunnan China) Abstract : The caryopsis of Dendrocalamus yunnanicus , which had been stored for 150 days at -20℃ and RH 15%, were used as the experimental materials, and aseptic clones were induced from them then used for propagation and in vitro conservation. The results showed that the best media for tufted-bud proliferation were MS + 6-BA 2 mg/L + NAA 0.2 mg/L, both sucrose and glucose contributed to the proliferation culture, and the best concentration was 3%. The most effective medium for rooting was MS + IBA 1 mg/L + NAA 1 mg/L, a single