Assessment of adhesion properties of novel probiotic strains to human intestinal mucus英文电子书.pdfVIP

International Journal of Food Microbiology 64 (2001) 119–126 www.elsevier.nl/locate/ijfoodmicro Assessment of adhesion properties of novel probiotic strains to human intestinal mucus a , * b ¨ ¨ a a Arthur C. Ouwehand , Elina M. Tuomola , Satu Tolkko , Seppo Salminen aDepartment of Biochemistry and Food Chemistry, University of Turku, Fin-20014 Turku, Finland b ¨ Novatreat Biotechnology Inc., Sepankatu 1, Fin-20700 Turku, Finland Received 11 March 2000; received in revised form 13 August 2000; accepted 29 September 2000 Abstract Potential new probiotic strains Lactobacillus brevis PEL1, L . reuteri ING1, L . rhamnosus VTT E-800 and L . rhamnosus LC-705 were assessed for their adhesion properties using the human intestinal mucus model. The effect on the adhesion of exposure to acid and pepsin and to milk were tested to simulate gastric and food processing conditions, and the effect of different growth media on adhesion was tested. The properties of the four strains were compared to the well-investigated probiotic L . rhamnosus strain GG. Three of the tested strains showed significant adhesion properties in the mucus model, while L . brevis PEL1 had intermediate adhesion and L . rhamnosus LC-705 adhered poorly. Pretreatment with different milks decreased the adhesion and low pH and pepsin treatment reduced the adhesion of all tested