兔延迟断蒂组织移植局部内皮素和一氧化氮变化的意义.docVIP

兔延迟断蒂组织移植局部内皮素和一氧化氮变化的意义 兔延迟断蒂组织移植局部内皮素和一氧化氮变化的意义 更新日期：2010-07-03 李少华　顾玉东　陈德松　陈亮　张高孟 　　【摘要】　目的　研究兔延迟断蒂组织移植局部内皮素(ET)和一氧化氮(NO)含量的变化及其与血管痉挛的关系。方法　用放射免疫方法测定局部血浆和动脉壁ET的的含量,用紫外—可见分光光度计测定局部血浆中NO2含量间接反映NO水平。结果　术后4小时,局部血浆及动脉壁ET含量增高非常显著(P0.01);术后8小时,局部血浆、动脉壁ET含量增高显著(P0.05)。术后4小时,局部血浆中NO含量下降非常显著(P0.01);术后16,24小时,局部血浆中NO含量增高非常显著(P0.01)。结论　术后16～24小时,延迟断蒂组织移植局部ET含量恢复正常,NO含量明显增高,是延迟断蒂组织移植血管危象发生率下降的原因,此时为断蒂的最佳时间。 　　【关键词】　组织移植　血管危象　内皮素　一氧化氮 Effect of Endothelin and Nitric Oxide Changes in Local Site of Tissue Transfer with Delayed Breaking Pedicle in Rabbits　　LI Shao-hua, GU Yu-dong, CHEN De-song, et al. Dept. of Orthopaedics, Changhai Hospital, Second Military Medical University, Shanghai 200433 　　【Abstract】　Aim　To study the changes of endothelin (ET) and nitric oxide (NO) in the local site of tissue transfer with delayed breaking pedicle and the relation with vasospasm in rabbits. ethods　The ET concentration of blood and local vascular wall were determined with the radio-immunoassay method. And the plasma NO2 levels in the local site of tissue transfer with delayed breaking pedicle, which reflected NO levels indirectly, were detected with Ultraviolet-visible (Uv-VIS) spectrophotometer. Results　The endothelin concentration of blood and local vascular wall increased awfully significantly at 4 hour after the operation (P0.01), and significantly at 8 hour (P0.05). The plasma NO levels significantly decreased at 4 hour after the operation (P0.01). But at 16 hour and 24 hour after the operation,the plasma NO levels increased significantly (P0.01). Conclusion　The recovery of ET concentration of blood and local vascular wall and the increase of the plasma NO at 16-24 hour after the operation are the cause of the reduced incidence of vascular crisis of tissue ransfer with delayed breaking pedicle, and the very time point is the optimum moment for pedicle breaking. 　　【Key words】　Tissue transfer　Vascular crisis　Endothelin　Nitric oxide 　　随着显微外科技术的发展,血管吻合技术不断精良,组织移植的成活率有了很大的提高。但是,在游离组织移