无创远端肢体缺血后处理对大鼠脑缺血再灌注损伤的影响.docVIP

精品论文 参考文献 无创远端肢体缺血后处理对大鼠脑缺血再灌注损伤的影响 李玲1通讯作者 李琼灿1 王云姣2 莫雪莹2 　　1长沙市中心医院麻醉科 湖南长沙 410004；2湘雅医院麻醉科 湖南长沙 410004 　　【摘 要】目的 研究无创性远端肢体缺血后处理对大鼠局灶性脑缺血/再灌注损伤的影响。方法 30只雄性SD大鼠（250-300g）随机分成3组（n=10），假手术组（Sham组）：不进行大脑中动脉梗阻；缺血再灌注组（I/R组）：采用大脑中动脉梗阻（MCAO）法建立大鼠局灶性脑缺血再灌注模型；无创远端肢体缺血后处理组（NRIPoC组）：在大脑中动脉缺血90min，再灌注即刻行无创远端肢体缺血5min，再灌注5min，反复3次。再灌注24h后对所有动物行神经功能学评分（NDS），然后取脑TTC染色测定脑梗死容积；HE染色观察脑组织形态；Nissl染色测定存活的神经细胞。结果 NRIPoC组较IR组24h NDS评分降低，但无显著差异（Pgt;0.05），脑梗死容积百分比显著减少（Plt;0.05），缺血侧皮层半影区存活的神经元数量显著增加（Plt;0.05），且半影区神经细胞层次较为清楚，形态较为完整，间质水肿减轻。结论 无创远端肢体缺血后处理可减轻大鼠局灶性脑缺血再灌注损伤。 　　【关键词】肢体；缺血后处理；缺血再灌注损失；无创 　　【Abstract】 Objective To observe the protective effects of noninvasive limb remote ischemic postconditioning against ischemic-reperfusion injury in MACO rats.Methods Thirty adult male Sprague-Dawley rats were divided randomly into three groups（n=10，each）：Sham group，I/R goup and NRIPoC group.The rats in Sham group only were isolated the vessels.The rats in I/R and NRIPoC group were received right middle cerebral artery occlusion（MCAO），and the rats in NRIPoC group were accepted 3 cycles of 5 minutes reperfusion followed by 5 minutes ischemia on right hind limb at the beginning of reperfusion in middle cerebral artery.At 24h after cerebral reperfusion，the neurologic deficit scores（NDS）of the rats in three groups were evaluated by the methods of Longarsquo;s.Five rats of each were then killed to measure their cerebral infarct size by TTC staining；and another five rats were randomly selected to observe the tissue morphology by HE staining，detect the survival nerve cells by Nissl staining.Results The NDS in NRIPoC group was lower than that in I/R group，but there was no significant difference between two groups（Pgt;0.05）.The brain infarction volume in NRIPoC group decreased significantly than that in I/R group（Plt;0.05）.The neurons in penumbra cortex in NRIPoC group were more clear and integrated than that in I/R group.Contrarily，the quantity of neurons was also more but the interstitial edema w