胶原聚丙烯酸互穿网络pH敏感水凝胶研究.pdfVIP

胶原/聚丙烯酸互穿网络pH 敏感水凝胶∗ 李凤娟，程庆甦，沈姝婕，穆畅道 四川大学 化学工程学院制药与生物工程系，成都，610065 摘要：环境敏感性水凝胶在生物物质分离提纯、酶的固定化及药物控制释放等方面有着 诱人的应用前景，因而近年来倍受关注。本实验采用生物相容性和生物可降解性都良好的胶 原和具有明显pH 敏感性的聚丙烯酸水凝胶在交联剂Bis 作用下制备互穿网络水凝胶，研究 了最佳制备条件及不同配比的水凝胶的溶胀动力学、pH 敏感性及pH 溶胀-退胀特性，并利 用傅里叶变换红外光谱法(FTIR)和示差量热扫描法(DSC)对其结构进行表征。结果显示：当 w(胶原) ︰w(PAA)= 2 ︰1，交联剂浓度为21mmol/L ，氧化还原体系浓度为15mmol/L 时，所 制备的水凝胶有最大的溶胀比66g/g，并且在13min 吸水率可达93% 以上；水凝胶有明显的 盐敏特性、pH 敏感性及pH 溶涨-退涨可逆性良好。FTIR 和DSC 结果显示, 水凝胶的制备 过程中保持了胶原的三股螺旋结构，且胶原与丙烯酸发生了交联，使胶原的结构更加有序。 水凝胶的吸热峰比胶原的吸热峰提高了49.2 ℃，水凝胶的热稳定性有了很大的提高，从而扩 大了该材料的应用范围。 关键词：胶原；丙烯酸；互穿网络；水凝胶；pH 敏感 Interpenetrating polymer network pH sensitive hydrogels based on collagen and polyacrylic acid Abstract: Recently, considerable research attention has been focused on the environment-sensitive hydrogels based on their great potential applications in many aspects, such as biomaterials separation and purification, enzyme immobilization and drug delivery system, etc. In the experiment, Interpenetrating polymer network (IPN) hydrogels were prepared by collagen with PAA in the presence of Bis as a crosslinking agent. The conditions of synthesis, such as mass ratio of monomer, the concentration of redox system and Bis and the process of gel fraction, were investigated. The molecular structure of the hydrogels was studied by Fourier Transform Infrared Spectroscopy(FTIR) and their thermal behavior was investigated by Differential Scanning Calorimetry(DSC). As a result, the hydrogel had the best swelling ratio 66g/g which was synthesized on the condition that the mass ratio of collagen and PAA was 2 ∶1, The concentration of crosslinking was 21mmol/L, and the redox system was 15mmol/L. The hydrogel represented good swelling behavior and the swelling ratio got up to more than 93% within 13min. The ∗基金项目：国家自然科学基金项目）；教育部“新世纪优秀人才支持计划” （NCET