氯诺昔康对大鼠骨质疏松性骨折愈合过程及转化生长因子β1的影响.docVIP

精品论文 参考文献 氯诺昔康对大鼠骨质疏松性骨折愈合过程及转化生长因子β1的影响 郑振雨 黄艳芬 朱明明 李静（新乡医学院第三附属医院 河南新乡 453003） 　　【摘要】目的 探讨氯诺昔康对趋势大鼠骨质疏松性骨折愈合过程中转化生长因子（TGF-beta;1）的影响。方法 选用8月龄雌性SD大鼠60只，去卵巢方法 建立骨质疏松模型，将大鼠制成左股骨骨折模型，随机分为对照组和实验组，术后给予实验组氯诺西康肌注，对照组给予同体积0.9%氯化钠注射液肌注，共10 d。术后在不同时间段处死，骨痂进行HE染色、TGF-beta;1免疫组织化学染色及图像分析。结果 HE 染色：实验组软骨生成及软骨内成骨过程较对照组延迟；TGF-beta;1免疫组织化学染色：实验组在各时间段TGF-beta;1的吸光度（IA）值均弱于对照组，差异有统计学意义。结论 氯诺昔康有可能通过抑制TGF-beta;1的表达，进而干扰骨质疏松性骨折的愈合过程。 　　【关键词】骨质疏松性骨折愈合 氯诺昔康 转化生长因子-beta;1 　　【中图分类号】R965 【文献标识码】A 【文章编号】1672-5085（2013）40-0037-02 　　Effects of lornoxicam on expression of TGF-beta;1 during the healing process of osteoporotic fracture in rats 　　ZHENG Zhengyu*，LI Chunjuan，ZHU mingming，LI Jing 　　(* The Third Affiliated Hospital of Xinxiang Medical College，Xinxiang，453003，China) 　　【Abstract】Objective To investigate the effects of Lornoxicam on expression of TGF-beta;1 during the healing process of osteoporotic fracture in rats. Methods 8-month-old female SD rats were ovariectomized to establish osteoporosis model,Fracture healing model was established by osteotomy on left femur. Sixty model rats were randomized into two groups: 30 for control group, and 30 for Lornoxicam group. Lornoxicam group was given daily by intramuscular injection at dose of 0.2 mg/kg per day for 10 days, while control group was given an equivalent volume of saline. The rats were sacrificed in batches at 1, 4, 8 weeks after operation, and the callus formation was examined with histological and immunohistochemistry assessment. Results Our analysis revealed delayed bone formation in Lornoxicam group. The Lornoxicam group showed lower integral optical density of TGF-beta;1 protein expression. Conclusion Lornoxicam may delay the osteoporotic fracture healing by inhibiting TGF-beta;1 expression. 　　【Key words】Osteoporosis；Fracture healing；Lornoxicam；Transforming growth factor beta 　　骨质疏松症是一种全身性系统性骨病，其特征是骨量下降和骨细微结构的破坏，使骨的脆性增加，轻微的创伤即容易发生骨折。骨质疏松性骨折是OP 的最严重后果，延迟恢复时间，影响手术