高流速下阴离子交换超大孔晶胶介质对ATP的层析分离特性研究.pdfVIP

高流速下阴离子交换超大孔晶胶介质对ATP 的层析分离特性 沈哲明 沈绍传 贠军贤 姚克俭1 （浙江工业大学化学工程与材料学院，绿色化学国家重点实验室培育基地，浙江杭州 310032 ） 摘要：接枝了DMAEMA 的阴离子交换超大孔晶胶介质作为一种新型分离介质具有传质阻力小等特点，允许在高 流速条件下快速分离生物物料。所用的晶胶介质在1 mL/min 到15 mL/min 的流速范围内，等板高度保持稳定， 渗透率达到4.1×10-12 2 m 。考察了5cm/min 和10cm/min 流速下ATP 在阴离子交换超大孔晶胶介质上的层析分离特 性，并与流速2cm/min 时的情况进行对比。结果表明，该阴离子交换超大孔晶胶有潜力成为ATP 高速分离的合 适介质。 关键词：阴离子交换超大孔晶胶；DMAEMA ；ATP ；吸附；高流速 Adsorption characteristics of ATP on anion-exchange chromatographic beds of cryogel at high flow velocities Shen Zheming, Shen Shaochuan, Yun Junxian, Yao Kejian （College of Chemical Engineering and Materials Science, Zhejiang University of Technology, State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, Hangzhou 310032, Zhejiang, China ） Abstract ：As a novel separating medium, anion-exchange supermacroporous cryogel with grafted polymer brushes of N,N-dimethylaminoethyl-methacrylate has the advantage over conventional of low flow resistance, allowing rapid separation at high flow velocities. In this work, the plate height (HETP) of anion-exchange supermacroporous cryogel is steady under different flow velocities between 1 to 15 mL/min and the permeability -12 2 of cryogel is 4.1×10 m .We compared the adsorption characteristics of ATP using cryogel at the flow velocities of 5 and 10 to those at 2 cm/min .The results showed that this type of cryogel is a potential matrice to be applied to purify ATP at high flow velocities. Key words ：Anion-exchange cryogel; N,N-dimethylaminoethyl-methacrylate; adenosine triphosphate; adsorption; high flow velocity 联系人：姚克俭，电话：0571，E-mail ：yaokj@zjut.edu.cn 第一作者：沈哲明（1984 -），男，浙江杭州人，硕士研究生。 基金项目：浙江省自然科学基金项目（No. Y405054 ，Y40