面包乳杆菌(Lactobacillus panis)C-M2细菌素的分离纯化及特性研究.docxVIP

面包乳杆菌（Lactobacillus panis）C-M2细菌素的分离纯化及特性研究胡彦新1，2，夏秀东1，刘小莉1，李 莹1，王 英1，董明盛2，周剑忠1，2*(1.江苏省农业科学院农产品加工研究所，江苏 南京 210014；2南京农业大学食品科技学院，江苏 南京 210095)摘要：本实验对面包乳杆菌C-M2所产的新型细菌素进行分离纯化和特性研究。通过乙酸乙酯萃取、阳离子交换层析和半制备液相三步分离纯化该细菌素。最终，细菌素的比活达到5044.96 AU/mg，纯化倍数为89.8倍，但回收率仅为0.35%。通过LC-MS/MS分析，该细菌素的分子量为863.52 Da，氨基酸序列为MVKKTSAV，它是一种新型的Ⅱ类细菌素。该细菌素具有广泛的抑菌谱，可以抑制革兰氏阳性和阴性的食品腐败菌。该细菌素对热和pH稳定，即使在121 ℃下灭菌15min,仍保留82.1%的抑菌活性，在pH 6下保留85.6%的抑菌活性。它能被多种蛋白酶失活，不能被脂肪酶和淀粉酶失活，这些结果表明该细菌素具有作为食品生物防腐剂的潜在应用价值。关键词：面包乳杆菌；细菌素；分离纯化；抑菌特性Purification and Characterization of the bacteriocin produced by Lactobacillus panis C-M2Hu Yanxin1,2, Xia Xiudong1, Liu Xiaoli1, Li Ying1 Wang Ying1, Dong Mingsheng2, Zhou Jian-zhong1,2*(1. The Research Institute of Agricultural Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2. College of Food Science and Technology, Nanjing Agriculture University, Nanjing 210095, China)Abstract：The present study aims to purify and characterize a novel bacteriocin produced by Lactobacillus panis C-M2. The bacteriocin was purified through extracted by ethyl acetate, cation exchange chromatography and semi-preparative high-performance liquid chromatography (RP-HPLC), respectively. Finally, the specific activity reached 5044.96 AU/mg and the purification fold was 89.8, but the recovery was only 0.35%. The bacteriocin has a molecular mass of 863.52 Da and the N-terminal sequence was MVKKTSAV by LC-MS/MS, showing low similarity with other class II bacteriocins reported before. It showed good stability to heat and pH, it retained 82.1% antibacterial activity even after exposure at 121 °C for 15 min and retained 85.6% under pH 6. The bacteriocin could be totally inactivated after being treated with protease, but it was not sensitive to lipase and amylase. These results indicate the potential of the bacteriocin for the application in the food preservation.Key words: Lactobacillus panis; Bacteriocin; Purification; antimicrobial characteristic中图分类号：R996.1 文献标识码：A 文章编号：细菌素