炎症介质对高血压患者颈动脉内膜中层厚度的影响.docVIP

精品论文 参考文献 炎症介质对高血压患者颈动脉内膜中层厚度的影响 （1.达州市中心医院心内科 四川 达州 635003） （2.四川大学华西医院心内科 四川 成都 610041） 【摘 要】目的：研究炎症介质IL-6、TNFalpha;对高血压患者颈动脉内膜中层厚度(intima-media thickness, IMT)的影响，探讨其可能的调控机制。方法：以至少一侧颈动脉IMTge;0.9mm为颈动脉内膜中层增厚的标准，将60名高血压患者分为颈动脉IMT正常组（n=32）和增厚组(n=28)。采用逆转录-PCR(reverse transcription-PCR, RT-PCR)方法测定单核细胞中IL-6、TNFalpha;及PPARgamma;的mRNA表达。结果：(1)与颈动脉IMT正常组相比较，IMT增厚组的IL-6和TNFalpha;mRNA表达升高 (二者Plt;0.001),而PPARgamma;mRNA表达降低(Plt;0.001)。(2)随着IL-6和TNFalpha;mRNA表达的升高，颈动脉IMT相应增加。（3）IL-6、TNFalpha;的mRNA表达与PPARgamma;mRNA的表达呈负相关(r1=-0.627，r2= -0.614，二者Plt;0.001)。结论：炎症介质IL-6、TNFalpha;的表达水平可影响高血压患者颈动脉内膜中层的厚度，这一作用可能与PPARgamma;参与炎症的调控有关。 【关键词】炎症；颈动脉内膜中层厚度；高血压；PPARgamma; 【中图分类号】R544.1 【文献标识码】B 【文章编号】1003-5028（2015）6-0609-02 【Abstract】Objective: To study the effect of inflammatory markers IL-6, TNFalpha; on carotid arterial intima-media thickness(IMT) in patients with hypertension, and explore the potential mechanisms of inflammatory regulation further. Methods: A total of 60 hypertensive patients were recruited. Carotid IMTge;0.9mm at least one side was defined thickened IMT, and thus all patients were assigned to normal IMT group(n=32) and thickened group (n=28), according to whether they had thickened carotid IMT or not. mRNA expression of IL-6, TNFalpha;, PPARgamma; in peripheral blood monocytes were performed by means of reverse transcription-PCR(RT-PCR). Results: (1) compared to that in normal IMT group, both mRNA of IL-6 and TNFalpha; in thickened group increased (both Plt;0.001), whereas mRNA of PPARgamma; decreased (Plt;0.001). (2) Carotid IMT increased with the elevation of IL-6 mRNA and TNFalpha; mRNA. (3) Both mRNA of IL-6 and TNFalpha; negatively correlated to that of PPARgamma; (r1= -0.627, r2= -0.614, respectively.Both Plt;0.001). Conclusions: Inflammatory markers IL-6, TNFalpha; are involved in the thickening of carotid arterial intima-media in hypertensive patients, which may be mediated by down regulation of PPARgamma;. 【Key words】inflammation; caro