基因技术定性测定转基因油脂研究.pdfVIP

基因技术定性测定转基因油脂 刘国琴韩立娟韩立鹏 (涮南1i、№火学粮油食晶学院，郯州450052) 攘要：本沦文综述了测窿辚鏊因油腻的蕊婺性翔玩年来熬阑泣逝憔测定转越陶走嫒湘脂的技术一普通Pc_R技术、 盘，多鼹盹R挂术、PCR-ELISA和寓时荧光雕R技术憋}匕较痰用的三种方法。琢此之外。述分移亍‘了油册加工过程 巾物理赫炼方法和纯掌精炼方法对熬吲注定性溺宛转蘩嘲油晒外源黧因D黼测定缩粜的影晌，滚意涮定结鬃的假 阴性，以钝弓I越缓蹦。这为避～步快而准确的定性溺滋转撼因油脂掘供参暂。 荧铤词t食晶科擘t麓瓯技拳：灞迩t转錾闲油鹏 Detectionof OilGenetic Qualitative Transgenicby Technology LIU Guoqin，HANLijuan，HANLipeng atHenan of 《GrainCollege UniversityTechnology,Zhengzhou450052) summarizedthe of oiland and Abstract：This paper significancedetectingtransgenieadvantages ofthe todetect oilwhichincluded：the disadvantagesgenetictechnologiestransgenicsoybean ordinary PCR PCR PCR-ELISA PCR-GeneScan technology,the multi#etechnology,thetechnology,the real—timefluorescence陀Randthe inrecent technology testingtechnology technology,the micro—array PCR andthereal—time technology,PCR-ELISA years．Comparatively，themultiple technology fluorescencePcR werethe threemethods．Inaddition．theofthe technologyquitepractical impact to resultsof methoddetectoils DNAcaused andchemical genetic exogenousgerm byphysicalrefining oil was ofthe 8Sto in results，SOavoid refining processinganalyzed．Attentionfalse-negative the for