四川山地乌骨鸡、颐康鸡IL2与γ干扰素基因克隆测序及真核表达质粒的构建研究.pdf

中国畜牧兽医学会禽病学分会禽流感及免疫抑制病论坛论文集 四川山地乌骨鸡、颐康鸡IL一2与吖干扰素基因 克隆测序及真核表达质粒的构建 胡慧琼4王红宁15周生黄勇柳萍徐永莉 (1．四川农业大学动物预防医学生物工程实验室，四)ll雅安625014) (2．四川大学生命科学学院，四川成都望江路29号610064) I双 酶切后，插入同样经HindlII和Kpn 子佐剂PCIFN—Y。该分子免疫佐剂PCIFN—y可作为禽类疫苗的免疫增强剂，’为进一步研究奠定基础。 关键词：鸡y干扰素鸡白细胞介素2序列测定真核表达质粒 andConstructionofRecombinant VectorofChickeninterferon--Yandinterleukin．．2 Cloning EukaryaticExpression proteingene Abstract：Abstract：Wethe basedonchickeninterferon—YinGenebank．andthenShandi designedprimers chickeninterferon—Ywas recerse chain Wugu amplifiedby transcription—polymerase blood stimulatedwithConA．ThewasclonedintoPMD18一Tvectorandthen periiaherallymphocytes gene the of resultshowedthat clone IFN—y ofclone ofIFN—ycDNAwas sequenced．The fragment length fragment frameofcloned consistedof ofclone ofIL一2 549 CHIFN—V bp，theopenreading 495bp。thelength fragment frameofclonedCHIL一2consistedof the with cDNAwas reading 432bp，Thendigestedgene Hind 530bp，theopen I．insertedinto wasalso with I．The digestedHindlII、Kpn III、Kpn pcDNA3．1(+)which Eukaryatic VectorofChickeninterferon—Y was Canusedasanimmune gene(PCIFN-Y)constructed．pClFN—Y for pourlty． interferon—Y Vector words；Chickengene；sequencing；EukaryaticExpression Key Y干扰素主要由活化T细胞和NK细胞产生，所