Bt毒蛋白免疫金标试纸条的制备与应用研究.pdf

Bt毒蛋白免疫金标试纸条的制备与应用 何素平，赵静，羿国香。刘威。邓艾兴．南铁责，李召虎，何钟佩，王保民 (中国农业大学棉花研究中心，中国农业大学农学与生物技术学院，北京100094) 摘要：利用胶体金标记的苏云金杆菌HD-73毒蛋白多克隆抗体，HD-73毒蛋白多克隆抗体和羊抗 鼠Igg制备了Bt毒蛋白免疫金标试纸条。试纸条的最低检测限为37．5ng·mL～。利用本试纸条对 四个棉花品种进行检测，结果与国外试纸条和生物测定结果一致。本试纸条可用于Bt抗虫棉及其种 子纯度的快速鉴定。 关键词：肢体金；试纸条；Bt抗虫棉；多克隆抗体 ofa forDetectionof Bt-cotton DevelopmentDipstickAssay Transgenic Research and Agricultural (Cotton Center，CollegeofAgronomyBiotechnology，ChinaUniversity，Be蚵ng 100094，China) of have a for detection in Bt·cotton．IIl Abstract：We developeddipstickassayqualitative CrylATransgenic the ofBacillus toxin tocolloidal strip．thepolyclonalantibody thurlnglensisproteinⅧnotonlycoupled gold also for usedasthe coatedOHthenitroceHulosemembranethetest anti-rabbit detector，but line，and goat washlottedonthenitrocellulosemembraneforthecontrol tothecemmercial．Btde- antibody line。Compared to tection evaluationofthis was wasshownbe Kit，the dipstickassayperformed．Thestrip ofhi出specificity of correlatedwiththatofthethecommercialBtdetection thelimitdetectionfor closely Kit，and Bt·protein inthis Was．Theseresults the for Bt-cottoninfield assay dipstick suggestpossibilitydetectingtransgenic and or Bt—cotton and the sereeningdifferentiatingtransgenienon．transgenieseed，simplyrelia