生物技术前沿动态-肖璐 4.ppt

http://bunseiserver.pharm.hokudai.ac.jp/gihou/knockout.html Chimeric mouse The brown fur comes from ES cells injected into the blastocyst of an albino(白化体)mouse Some Examples of Knockout Mice p27 knockout mouse is bigger than the control This is not due to obesity, but the skeletal structure is increased in size (everything about the mouse is larger) http://www.bioreg.kyushu-u.ac.jp/saibouE.html p27 knockout mouse http://www.bbc.co.uk/science/genes/gene_safari/wild_west/bigger_and_better02.shtml GDF8 (Myostatin) knockout mouse Over twice the muscle mass of a wildtype mouse normal knockout Naturally Occurring GDF8 Mutants /victoriatimescolonist/story.html?id=67f15c17-2717-4022-bb76-1b982456e793k=94653 http://www.bbc.co.uk/science/genes/gene_safari/wild_west/bigger_and_better02.shtml FGF5 knockout mouse has long, angora-like hair http://www.med.uni-jena.de/ivm/deutsch/method/method_7.htm Clones and Cloning /community/health-science/scientificcommunity/index2.html Dolly as a lamb with her surrogate mother Dolly, First Mammal Cloned From an Adult Cell Dolly, as an adult /cloning3.htm Somatic Cell Nuclear Transfer What Has Been Cloned So Far? Somatic Cell Nuclear Transfer Sheep, Goat, Mouse, Rabbit, Cattle (domestic wild), Pig, Horse, Mule, Dog, Cat (domestic wild), Deer Embryo Splitting (Twinning) Sheep, Cattle, Primate (Rhesus) Cat Clone Donor Surrogate mother with clone (CC) Out of 87 implants only CC survived to birth ???????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? /news/science/2003-01-21-cloned-cats_x.htm Donor Clone Rainbow CC Cloned transgenic cat containing red fluorescent protein /story/_a/glowing-pig-passes-genes-to-piglets/20080109143909990001?ncid=NWS00010000000001 Transgenic Clones /news/2003/05/0529_030529_muleclone.html Idaho Gem, first cloned mule 1st try 134 implants 2 pregnancies, both failed