马钱子配伍白芍减毒增效作用的研究.pdfVIP

提 要 目的：本课题选择不同配伍比例的马钱子配伍 白芍煎剂，观察其对毒性、止痛作 用的影响，探讨其止痛作用机理。 方法：本课题通过急性毒性实验及高效液相色谱分析观察马钱子配伍 白芍对马钱 子毒性的影响；通过小鼠热板法与小鼠扭体法观察其对止痛作用的影响；通过对佐剂 关节炎所致大鼠足肿率、大鼠免疫器官系数及大鼠血清中丙二醛、超氧化物歧化酶、 一氧化氮的测定探讨马钱子配伍白芍镇痛、抗炎作用机理。 结果：本课题通过实验研究，证实马钱子配伍 白芍能够降低马钱子毒性，增强马 钱子镇痛、抗炎效果，二者配伍有减毒增效作用。其镇痛机理是通过抗 自由基损伤、 抗炎和保护细胞，起到镇痛作用。 结论：本课题认为马钱子配伍白芍减毒增效的配伍比例以 1：6 为宜，同时本课 题也验证了中药性味配伍理论的正确性。 关键词 马钱子；白芍；配伍；减毒增效 Experim ental R esearch of D ecreasing Toxicity and Increasing Efficacy on the C om patibility of Nux V om ica and Paeonia lactiflora Speciality：Traditional Chinese M ateria m edica A uthor：liu ye Tutor：Professor Tang Y ingxue A bstract Objective: this project choose different com patibility proportion of sem en strychni com patibility of radix paeoniae A lba decoction to observe its effects on the toxic and analgesic effect of and discussion on its m echanism of pain. M ethod: this issue through acute toxicity and high perform ance liquid chrom atographic analysis of toxicity effect of com patibility of N ux Vom ica sem en strychni glucosides. By hot-plate and m ice in m ice by tw isting effect observation of its effect on the pain. A djuvant-induced arthritis rats induced by foot through the sw ell rate, coefficient of imm une organs of rats and rat serum M DA and SOD , NO discussion on the determ ination of the com patibility of sem en strychni glucosides of analgesic and anti-inflam m atory effects m echanism . Results: theoretical and experim ental study on this subject by, certified com patible reduces glucosides of sem en strychni sem en strychni toxicity, enhanced sem en strychni analgesic and anti-inflam m atory effects, com patibility w ith reducing poison and increasing effect of the tw o. Its analgesic, anti-inflamm atory m echanism is through the free radic