体内药物分析——固相萃取.pptVIP

Solid Phase Extraction 目 录 pharmaceutical Analysis in Biological Samples principle of SPE Procedure advantages and disadvantanges application of SPE Definition and Application of SPE  The process of the Biopharmaceutical analysis: 1、Sample collection 2、Standard solution configuration 3、Plasma sample processing 4、Chromatographic conditions and system suitability test 5、Method validation 6、Determination of plasma concentration 1.Definition and Pocess of pharmaceutical Analysis in Biological Samples(体内药物分析的定义及过程) Solid Phase Extraction (Solid Phase Extraction, referred SPE) is the use of selective adsorption and selective elution liquid chromatography separation principle of the separation of the sample, a sample purification and enrichment before processing techniques. By the liquid-solid extraction and liquid chromatography combined evolved. 2.Definition and Application of SPE Currently in the international arena, solid-phase extraction technology has been widely used in medicine, clinical blood drug concentration monitoring, forensic chemistry, before the inspection and quarantine, environmental protection, water quality, food industry sample handling. It is noteworthy that in the field of biotechnology and bioengineering, people began using solid phase extraction purification of biological samples were. Moreover, automated solid phase extraction device is also becoming more widely used, such as ASPEC XL series solid-phase extraction system Gilson company has become a testing center in many laboratories including the Olympic Games, including the necessary samples before processing machines 3.principle of SPE SPE is the use of liquid chromatography separation principle of selective adsorption and selective elution. The more common approach is to make the liquid sample solution through the adsorber, which retained the test substance, then choose the appropriate strength solvent rushed impurities and then eluted