[精品文档]刺槐心材腐朽菌的鉴定及其生物学特性.pdf

38 8 V o l. 38 N o. 8 20 10 8 JOURNA L O F NORTHEA ST FORESTRY UN IVER SITY A ug. 20 10 高国平 信 雯 郑雅楠 高 拓 祁金玉 张 伟 魏振宏 ( , , 110 16 1 通过形态学和分子生物学方法鉴定沈阳地区刺槐心材腐朽病病原菌 ( 编号为 P 1 , 并 室内培养条 件下, 采用生长速率法研究了温度pH 值光照碳源和氮源对病原菌菌丝生长和菌落形态的影响结果表明: 病 原菌P 1为槐生多年卧孔菌( P erennpi oria rob in iop h ila ( M urr. R yv. ; 菌丝生长适宜温度为 20 ~ 25 , 适宜pH 值为 5 ~ 6, 光照对菌丝生长无显著影响此外, P 1对碳源的利用以蔗糖为最佳, 氮源以酵母膏最佳 刺槐; 病原菌鉴定; 生物学特性; ITS rDNA S792. 26 Iden tification and B iologica l Characteristics of Locu st H eartw ood Rot Fung i/ G ao G uoping, X in W en, Zheng Y anan, G ao T uo, Q i J inyu , Zhang W e,i W en Zhenhong ( School of Forestry, Shenyang A gr icultural U n ive rsity, Shenyang 11016 1, P. R. Ch ina / / Journal o f N o rtheast F orestry U niversity. - 20 10, 38( 8 . - 60 ~ 62 H earwt ood ro t fungus ( P 1 of R obin ia p seudoacac ia in Shenyang w as identif ied by m orpho log ical and m olecu lar bio log ical m ethods. A nd the e ffects of tem perature, pH va lue, ligh t, and carbon and n itrogen resou rces on myce lium grow th and co lony m orphology w ere stud ied by g row th rate me thod. R esu lts show ed tha t P 1w as iden tified asP erennpi oria robin io p h il( M urr. R yv. T he optmi um temperature fo r the m ycelium grow th w as 20 ~ 25 degrees C, and the optmi um pH va lue w as w ith in the rang e of 5~ 6. Illum ination had no obv iou s effect on myce lium grow th. T he m yce lium grew best on m edia w ith cane sugar as ca rbon source o r yeast extract as nitrogen resources. K eyw ords R obin ia p seudoacac ia; Identif ica tion; B iolog ica l character istics; IT S rDNA (R obin ia p seudoacac ia G enbank, BLAST C lu stal X ( ver