胃癌患者外周血hTERTmRNA的表达及意义.pdfVIP

# 502# 2009 6 31 6 N ingxi M ed J, Jun12009, Vol 31, No16 : 1001- 5949( 2009) 06- 0502- 02 # 论 著# hTERT mRNA 1 2 2 2 2 1 1 魏建敏 , 刘新兰 , 刘奇伦 , 葛 箭 , 陈 萍 , 崔 洁, 丛芳圆 [] hTERT mRNA RT - PCR 4020 20 hT ERT mRNA hTERT mRNA ; hTERT mRNA CEACA 199(P 0. 05); hTERT mRNA CA 199(P 0. 05) RT - PCR hTERT mRNA [] hTERT mRNA; CEA; CA 199; 胃癌; 微转移 [] R392. 12 [] A Clinical significance on detectig hTERTmRNA in peripheral blood ofpatientswith gastric carcinoma WEI J ian - m in, et al. ( N ingx i M ed ic lUn iversity, Y inchu n 750004, Ch in ) [Abstract] Objective T o n lyze whether hTERT mRNA c n be used sm olecul r b iologic lm rker of pred icting m icrom et s- t sis in peripher l blood of p tien tsw ith g stric c rcinom . Methods Peripher l b lood of 40 p tientsw ith g stric c rcinom , 20 p tients w ith chron ic troph ic g stritis nd 20 he lthy c sesw ere detected by RT - PCR techn ique. Results Com p ring the p tients w ith chronic trophic g stritis nd he lthy people, the positive expression r te of hTERT mRNA in peripher l b lood of p tien ts w ith g stric c rcinom w s s ignific ntly incre sed; the positive expression r te of hTERT mRNA w s positively correl ted w ith the express ion of CEA nd CA199(P 0. 05) ; The positive express ion r te of hTERT mRNA w s h igher th n th t of CA 199 (P 0. 05). Conclution The expres- sion of hTERT mRNA detected by RT - PCR techn ique c n be used s predictive m rker for di gnosing m icrom et st sis in peripher l b lood of p tients w ith g stric c rcinom . [ eywords] hTER T mRNA; CEA; CA 199; Gastric carc inom a; M icrom etastasis (RT- PCR) 1. 2 方法 ( hTERT ) 1. 2. 1 RT - PCR hTERT