Sample Prep Made Easy英文资料.pdfVIP

Winter 1999/2000 How to Choose the Proper SPME Fiber A continuation of reports discussing SPME parameters, begun in the Fall 1999 SPME newsletter 1 The most common question we receive concerning SPME is, natu- rally, “Which fiber should I use for extracting my specific analyte?”. Recently, we evaluated the abilities of currently available SPME fibers to extract organic analytes of various classes and molecular weights. From the results, we hoped to make some general recom- mendations. First we focused on volatile analytes with similar size (molecular weight 58-89) and structure, but different functionalities (Table 1). We introduced these analytes into water at 1ppm each, extracted them, using 6 different fibers, then determined absolute responses for each analyte, extracted by each fiber. Table 1 lists the best fi- bers for extracting each of these analytes. Where possible, the three best choices are given. For extracting analytes with molecular weights of less than 90, re- gardless of functionality, the clear choice is the 85µm CarboxenTM/ polydimethylsiloxane (PDMS) StableFlexTM fiber. For nearly all of (contd. on page 2) P000144, 9970141 Table 1. Best SPME Fiber Choices for Extracting Analytes Analyte Mol. Wt. 1st Choice 2nd Choice 3rd Choice Acetone 58 Carboxen ™/PDMS Propanal 58 Carboxen/PDMS Isopropanol