稀有鮈鲫Dmrt基因家族13个成员的克隆与序列分析 Clone and Sequence Analysis of Thirteen Members of Dmrt Genes in Rare Minnow.pdfVIP
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稀有鮈鲫Dmrt基因家族13个成员的克隆与序列分析 Clone and Sequence Analysis of Thirteen Members of Dmrt Genes in Rare Minnow
第2卷第1期 生 态 毒 理 学 报 V01.2.No.1
Asian of
Journal 硅射.200}7
2007年3月 Ecotoxicology
稀有煞鲫Dmrt基因家族13个成员的克隆与序列
分析
张小艳,孙立伟,查金苗,王子健+
中国科学皖生态环境研究中心珂’境永艟学国象重点实验室,北京100085
个其有DNA结音馥力的缫守基穿——l}M结构域,对性剐央定张褴捌分化萁有调控功能。乖j翔箍并PCR,鼠稀有觞
化树,结果表明,稀有觞鲫基因缎存在多个Dmrt基因成员,该纂因在脊椎动物和非脊椎动物中具有高度保邻性,
是一种理想的环境内分泌干扰物研宠的分子撰型,在分子生态毒瑗学研究中具有很好辩应用前景。
关键词:稀有绚鲫;DM结构域;兼并PcR;毒理学
文章编号:1673—58卯(2007)l一088—06 文献标识码:A
中嘲分类号:R99,X503.225
Cloneand ofThirteenMembersofDmrtGenesinRare
SequenceAnalysis
Minnow
ZHANG
Xiao—yan,SUNLi—wei,ZHAJin—miao,WANGZi-jian+
Stale ofEnviroameatat ResearchCenterforEeo-EnvironmentalSciencea.
Key Chemistry.
Laboratory Aquatic
Chinese of 100085
AcademySciences,Beijing
2006
Received7November2006 9D∽ernbH
accepted
Mab一3of andDoubtesexof related
Abstract:Sex Doubtesex D融RTl
determininggenes c.egegans Drosophila。human gene
and containacoInlnonDNA motiftailedDM£Doublese墨andMab一3)
medaka姒一determininggene蝴iV/Y/DMY binding
ofwhich similar ofsexual DNAoftherareminnow
domain,bothregulate aspects development.Weamplifiedgenomic using
theDM anddetectedan band.Afterclonedinto MD-19vectorand
primers 140bp sequenced.
degenerate appmxJmately P
thirteen showed withtheDMdomain,BasedOnsimilaritiesofaminoacid oftheDM
sequenceshighhomology sequences
domain.Drnrt from weieincludedina tre
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