陆地棉基因家族的全基因组分析.pdfVIP

棉 花 学报 Cotton Science 2017 袁29 渊6冤 495―503 陆地棉 基因家族的全基因组分析 周娜 汪露瑶 张天真 胡艳* 京农业大学作物遗传与种质创新国家重点实验室袁南京210095冤 摘要院揖目的铱 是1 类保守的转录因子家族袁参与调控植物的开花时间和花器官发育遥 通过对 家 族进行全基因组学分析袁为深入研究 在棉花开花及花器官发育的分子调控机理提供基础遥 揖方法铱利用 HMMER 3.0 及pfam 种子文件鉴定棉花全基因组 基因袁结合其表达量进行偏向表达及聚类分析遥 揖结 果铱在陆地棉基因组中共鉴定发现100 个 基因袁分为11 个亚类遥 表达聚类分析显示袁棉花 基因 的表达模式大致可分为4 个不同的类群袁说明这类基因在棉花进化中出现了功能分化遥100 个 基因中袁 有12 个基因具有miRNA 靶位点遥揖结论铱研究结果显示 家族基因在棉花纤维中存在功能分化袁而且可 能受到miRNA 的调控袁这为进一步研究该家族基因的功能提供信息参考遥 关键词院陆地棉曰 基因曰功能分化曰miRNA 中图分类号院S562.03 文献标志码院A 文章编号院1002-7807(2017)06-0495-09 10.11963/ 1002-7807.znhy.201709 13 Zhou Na, Wang Luyao, Zhang Tianzhen, Hu Yan* ( 210095, ) [Objective] In plants, floral organ identity and the process of the transition from vegetative to reproductive development is regulated by gene family. Whole genome analysis is helpful to understand the evolutionary history of genes and provides the basis for an in-depth analysis of molecular mechanism of family in flowering and floral organ develop- ment in cotton. [Method] Whole genome identification of genes were carried by HMMER 3.0 and pfam gene bias ex- pression and were clustered based on the RNA-seq data. [Result] We identified 100 genes and classified them into 11 subfamilies, according to their phylogenetic relationships to the and genes. According to their expression profiles, genes were classified into four types. The genes